摘要
为了探讨鹅源新城疫病毒"自杀性"DNA疫苗的免疫效果,将鹅源新城疫病毒HN基因片段克隆到自杀性DNA疫苗载体pSCA1中,构建了表达HN基因的自杀性DNA疫苗质粒pS-HN。间接免疫荧光试验结果证实,该质粒转染BHK-21细胞后实现了HN基因在该细胞中的表达。将该疫苗和常规DNA疫苗(pc-HN)分别免疫鹅,结果表明,在诱导体液、细胞免疫和攻毒保护方面,pS-HN均优于pc-HN,这为进一步评价该疫苗的免疫保护效果奠定了基础。
To evaluate the immune efficacy of the suicidal DNA vaccine against goose-origin Newcastle disease,a suicidal DNA vaccine plasmid expressing HN gene of goose-origin Newcastle disease virus was constructed by inserting the HN gene fragment into the expression vector pSCA1,and named as pS-HN.The plasmid was then transfected into BHK-21 cells and the HN protein expression was demonstrated by indirect immunofluorescence assay.Animal immune experiment was conducted by inoculating goose with the pS-HN.The pc-HN,convention DNA vaccine expressing the same HN gene,pcDNA3.1（＋） and pSCA1 were used as control.The results revealed that pS-HN was superior to pc-HN in inducing humoral immune response,cellular immune response and have better protective rate against goose-origin Newcastle disease virus.The study laid a foundation for evaluating the protective efficacy of pS-HN.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第7期842-846,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)
广东省科技攻关(5500-E08020
2007A020100006)
公益性行业(农业)科研专项经费(200803020-08)
