摘要
目的探讨Ⅱ度烧伤创面愈合过程中角朊细胞间粘附分子-1(ICAM-1)的表达及其意义。方法应用免疫组织化学染色技术,对烧伤后不同时间Ⅱ度烧伤皮肤组织角朊细胞 ICAM-1的表达进行动态观察。8例健康人皮肤,6例烧伤病人正常皮肤及8例Ⅱ度烧伤愈合后增生性瘢痕组织为对照组。结果健康人正常皮肤基底层细胞 ICAM-1可低表达,烧伤病人正常皮肤基底层细胞ICAM-1可表达增强。Ⅱ度创面基底层细胞伤后1周内 ICAM-1表达轻度增高,2周内明显增强,2～4周稳定高表达,ICAM-1呈极性分布,新生上皮多层角朊细胞 ICAM-1表达显著增强,ICAM-1阳性细胞呈长柱状排列。结论Ⅱ度烧伤皮肤角朊细胞 ICAM-1表达增强可能与创基炎性细胞浸润相关,具有诱导角朊细胞增殖、迁移,加速上皮化的作用。
Objective To investigate the significance of changes in intercellular adhesion molecule- 1(ICAM-1)expression on keratinocytes(KCs)during wound healing of second degree burn.Methods With immunohistochemistry technique,the ICAM-1 expression in second degree burn wounds in patients were investigated at four different postburn periods.The normal skins from 6 burn patients(BNG),the normal skins from 8 healthy volunteers(HVG)and the scar tissues(SG)from 8 patients served as con- trols.Results There was poor expression of ICAM-1 on basal cells in normal skins of healthy volunteers, while the expression of ICAM-1 enhanced in normal skins of burn patients.On basal cells in second degree burn wounds,the expression of ICAM-1 slightly increased in the first week postburn,obviously enhanced in the second week,and in the third or fourth week,the expression continued to keep strong.In addition, the ICAM-1 in KCs showed polar distribution,and the expression of 1CAM-1 markedly enhanced on the multilayer KCs in neogenetic epithlium and the positive cells arranged columnarly.Conclusion The en- hanced expression of ICAM-1 on KCs in second degree burn wounds might be related to invasion of in- flammatory cells beneath wound.It might play a role in inducing proliferation and migration of KCs and accelerating epithelization.
