摘要
目的观察和验证正常大鼠骨骼肌组织是否存在有氧糖酵解的生理过程,烧伤早期和脓毒症状态下骨骼肌组织有氧糖酵解过程是否发生显著的变化。方法借助大鼠烧伤和脓毒症模型,建立伸趾长肌和比目鱼肌的充分供氧离体孵育系统,采用 NADH 荧光探针方法,检测骨骼肌组织细胞乳酸的产生量及其变化。结果在可靠的肌肉组织充分供氧离体孵育系统中,两种肌肉组织在烧伤后和脓毒症情况下乳酸产生量均较正常对照组有不同程度的增加;不同类型的肌肉组织有氧糖酵解的水平及其在烧伤后和脓毒症状态下有氧糖酵解过程的改变具有各自不同的特点。结论在烧伤和脓毒症情况下,骨骼肌细胞存在有氧糖酵解增强的病理生理过程,即组织缺氧并非细胞乳酸产生增加的唯一原因。对烧伤及脓毒症中骨骼肌有氧糖酵解及其发生机理的深入分析和研究,有重要的理论和临床意义。
Objective To verify if aerobic glycolysis exists in the muscle cells in normal rats and to analyze the changes in aerobic glyeolysis in the muscle cells in the early postburn period and in septic states.Method Using septic model of rats,we established the in vitro muscle incubation system with suf- ficient oxygen supply as well as the NADH fluoremetric method for the detection of trace amount of lactate in the samples.Extensor digitorium longus(EDL)and soleus muscles which represent two types of muscle fiber were studiedd.Results In the early postburn period as well as in septic states,the lactate production of muscle cells was significantly elevated as compared to the normal controls even though the muscles were incubated in a fully oxygenated media.The levels of aerobic glyoclysis,as well as its changes in postburn period and in septic states,vary depending on the difference in the fiber composition of the muscles.Con- clusion Muscle cells might develop a kind of metabolic enhancement which is referred to as aerobic gly- colysis rather than the metabolic defect which results from tissue hypoperfusion and hypoxia in the early postburn period as well as in septic states.This provides us a special insight to elucidate the mechanisms of the metabolic derangement in the early postburn period and in sepsis.
基金
军队医药卫生科研基金资助(编号:97H31)
