摘要
目的制定肃清丸质量控制标准。方法采用薄层色谱法对肃清丸中的金银花、玄参、当归、黄芪、地榆、甘草、连翘进行定性鉴别,采用高效液相色谱法测定肃清丸中绿原酸的量。色谱柱为LunaC18柱(250mm×4.6mm,5μm),流动相为乙腈-0.4%磷酸溶液(10:90),柱温30℃,体积流量为1.0mL/min,检测波长为328nm。结果金银花、玄参、当归、黄芪、地榆、甘草、连翘薄层色谱斑点清晰,重现性好,无干扰;绿原酸在5.4～54.0μg/mL线性关系良好,平均回收率为99.77%,RSD=1.59%(n=6)。结论所建立的定性和定量方法结果准确、可靠、重现性好,可用于肃清丸的质量控制。
Objective To establish the quality standard for Suqing Pills. Methods TLC was applied to identify Flos Lonicerae , Radix Scrophulariae , Radix Angelicae Sinensis , Radix Astragali , Radix Sanguisorbae, Radix Glycyrrhizae, and Fructus Forsythiae. HPLC was applied to determine the contents of chlorogenic acid in the prescription, in which Luna C18 column （250 min×4.6 mm, 5 μm）was used . The mobile phase consisted of acetonitrile -0.4% phosphoric acid （10 ： 90） solution, and the flow rate was 1.0 mL/min. Column temperature was at 30 ℃ and the detection wavelength was set at 328 nm. Results The spots on TLC plates were clear without interference in the blank reference. The linearity range of chlorogenicacid was 5.4-- 54.0 μg/mL, the average recovery of chlorogenic acid was 99.77%, and RSD was 1.59% （n=6）. Conclusion The established method is accurate, feasible and reproducible. It can he used for the quality standard control of Suqing Pills.
出处
《现代药物与临床》
CAS
2010年第3期214-219,共6页
Drugs & Clinic
