摘要
目的比较芳香新塔花Ziziphora clinopodioides不同提取物对大鼠急性心肌缺血、对乳鼠心肌细胞缺氧-复氧损伤的影响。方法通过体内注射垂体后叶素造成大鼠急性心肌缺血模型,观察急性心肌缺血大鼠Ⅱ导联心电图J点的变化,以及试药对超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)、丙二醛(MDA)的影响。体外培养原代SD乳鼠心肌细胞,建立心肌细胞缺氧-复氧损伤模型,用MTT法测定药物对心肌细胞存活率的影响,测定给药后MDA的变化。结果芳香新塔花石油醚部位和氯仿部位0.3g/kg显著抑制注射垂体后叶素所致心电图J点升高,对心率、SOD、LDH、MDA的影响不显著;乙醇提取物和氯仿部位能明显降低缺氧-复氧模型下心肌细胞培养液中的MDA浓度。结论芳香新塔花具有潜在的心肌缺血保护作用。
Objective To study the protective effects of ethanol extract and isolated fraction from Ziziphora clinopodioides Lam. on acute myocardial ischemia of rats and neonatal rat cardiomyocytes damaged by hypoxia/reoxygenation stress. Methods Acute myocardial ischemia of rats was induced by injection of pituitrin. Electrocardiogram （ECG） was recorded, and the levels of lactate dehydrogenase （LDH）, super-oxide dismutase （SOD） and the malondialdehyde （MDA） in medium were also tested. The neonatal rat cardiomyocytes were isolated and cultured in vitro to establish hypoxia/reoxygenation injury model. The viability of cardiomyocytes was measured by MTT method. Change of MDA content in medium were also determined. Results Petroleum ether fraction and chloroform fraction （0. 3 g · kg^-1） remarkably inhibited the raise of J point caused by pituitrin, and significantly decreased MDA content in the model of hypoxia/reoxygenation injury. Conclusion Z. clinopodioides has a potential protective effects on acute myocardial isehemia and neonatal cardiomyocyte damage induced by hypoxia/reoxygenation stress.
出处
《现代药物与临床》
CAS
2010年第3期194-198,共5页
Drugs & Clinic
基金
新疆维吾尔自治区科技基础条件平台建设项目(PT0708)
新疆维吾尔药实验室课题(xjys0207-2008-03)
关键词
芳香新塔花
药理作用
心肌缺血
心肌细胞
缺氧-复氧
Ziziphora clinopodioides Lam.
pharmacological effect
myocardial ischemia
cardiomyocytes
hypoxia/reoxygenation
