摘要
以槟榔花茶为试验材料,分别采用3种提取方法,即:①100℃沸水浴提取3次,每次30min;②超纯水常温提取3次,每次6h;③95%的乙醇常温提取3次,每次6h。研究采用不同提取方法对应所得的槟榔花提取物对Fe2+的络合能力、Fe3+的还原能力和DPPH自由基的清除作用;并测定每种提取物的总酚含量,以Fenton反应为模型,研究不同提取物对羟基自由基诱导2-脱氧核糖降解的保护机理。结果表明,以第1种提取方法(100℃沸水浴提取3次,每次30min)所得的提取物抑制脱氧核糖降解和清除DPPH自由基的能力较强,总酚含量较高,但其对Fe2+的络合能力和Fe3+的还原能力相对较弱。
Areca inflorescences tea was extracted with the following three different methods： boiling water for 30 minutes, ambient water for 6 hours and 95% ethanol for 6 hours. The protective effects including iron （Ⅱ） chelation, reducing power and DPPH radical scavenging activity and Fenton chemistry system on hydroxyl radical-mediated 2-deoxyribose degradation of the extracts were assayed. The total phenolic contents of various extracts were also measured. The results revealed that the inhibitory effect on 2- deoxyribose degradation, DPPH-scavenging activity and total phenolic content of the boiling water extract was higher than the ambient water extract and ethanol extract . But its iron （Ⅱ） chelation activity and reducing power were weaker than that of the others.
出处
《热带作物学报》
CSCD
2010年第6期949-953,共5页
Chinese Journal of Tropical Crops
基金
海南省重点科技计划项目(No.090138)资助
关键词
槟榔花
提取物
羟基自由基
脱氧核糖降解
Areca inflorescence
Extracts
Hydroxyl radical
2-deoxyribose degradation
