摘要
目的观测静脉输注人、猪、豚鼠全血对大鼠存活及内脏功能的影响，为今后研究供体特异性输血诱导异种免疫耐受奠定基础。方法大鼠阴茎背静脉输注人、猪、豚鼠全血，每只大鼠每天１ｍｌ。输人血的４５只大鼠平均分为Ⅰ、Ⅱ、Ⅲ组，其相应输血总量分别为１ｍｌ，６ｍｌ，１０ｍｌ，观察大鼠一般情况，存活率，并在输血后第１０天，第６０天分别处死７只、８只，行ＰａＯ２、ＰａＣＯ２、Ａ－ａＤＯ２、ＧＰＴ、ＧＯＴ、ＴＰ、ＬＤＨ、Ｃｒ、ＢＵＮ以及心、肝、肾、肺、脾脏、胃、血管病理形态检查。输注猪、豚鼠血大鼠仅观察一般情况，存活率。结果输注人、猪、豚鼠血大鼠精神好，全部存活。输人血前及输血后第１０天，第６０天大鼠组及各组间ＰａＯ２、ＰａＣＯ２、Ａ－ａＤＯ２、ＧＰＴ、ＧＯＴ、ＴＰ、ＬＤＨ、Ｃｒ、ＢＵＮ比较差异无显著性（Ｐ＞０．０５）。心、肝、肺、肾、脾、胃、血管病理检查无异常发现。结论大鼠接受异种全血静脉输注不会损伤大鼠内脏功能。
Objective In order to demonstrate whether donor specific blood transfusion induces tolerance in murine xenografting. Methods We first studied effect of xenogenic blood transfusion on visceral functions of rats. In this study, human, pig and guinea pig were used as blood donors and the outbred wistar rats as the recipients. Forty five recipient rats transfused with human blood were randomly divided into group Ⅰ,Ⅱand Ⅲ. The total human blood volume for group Ⅰ,ⅡandⅢ were 1ml, 6ml and 10ml, respectively. One ml blood in volume was adminstered to one rat via the vein each day and the rat survival, the serum values of PaO 2, PaCO 2, A aDO 2, GPT,GOT,TP, LDH,BUN and Cr as well as the pathological changes of heart,liver,lung,kidney,spleen,stomach and the vascularity of the rats before and after human blood transfusion were evaluated;whereas the rats transfused with pig and guinea pig blood were used for observing their survivals only. Results All rats survived well after transfusion with human. Pig and guinea pig blood, there were no statistical difference about both the serum values of PaO 2, PaCO 2, A aDO 2, GPT, GOT, TP, LDH, Cr, BUN( P >0 05) and the pathological changes of heart, liver, kidney, lung, spleen, vascularity, stomach of the rats before and after human blood transfusion. Conclusions These results indicated that xenogenic blood transfusion did not affect rat survival and visceral functions and could be used to study xenogenic tolerance induction in murine xenotransplantation.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1999年第1期51-54,共4页
Chinese Journal of Microbiology and Immunology
关键词
异种
输血
大鼠
内脏功能
Xenogenic blood transfusion Rat Visceral function
