摘要
用荧光素酶报导基因，发现ｃｅａ启动子在ＣＥＡ阳性的肺腺癌细胞Ａ５４９中的活性是在ＣＥＡ阴性的宫颈癌细胞Ｈｅｌａ中的１６倍．构建了重组表达质粒ｐＣＥＡｔｋ，ｃｅａ启动子控制效应基因单纯疱疹病毒胸苷激酶基因（ＨＳＶｔｋ）的表达．转染了质粒ｐＣＥＡｔｋ的Ａ５４９细胞对甘昔洛韦（ＧＣＶ）的敏感性是未转染细胞的８６５倍，而转染了ｐＣＥＡｔｋ的Ｈｅｌａ细胞则仍保持对ＧＣＶ的抗性．
By using luciferase reporter gene, we found that promoter of carcinoembryonic antigen (CEA)gene exhibit 16 times high activity in CEA producing lung cancer cells, A549 than in nonproducing cells, Hela. We also constructed a recombinant expression plasmid pCEATK, in which CEA promoter drives the effector gene, thymidine kinase gene of Herpes Simplex Virus (HSVTK). A549 cells transfected with pCEATK became 865 times more sensitive to ganciclovir (GCV) than the control cells. However, Hela cells transfected with this plasmid remained resistant to GCV. These data indicate the potential for targeted gene therapy using the CEA promoter against CEA producing tumor cells, such as lung cancer cells.
出处
《武汉大学学报（自然科学版）》
CSCD
1999年第2期215-219,共5页
Journal of Wuhan University(Natural Science Edition)
基金
国家自然科学基金
湖北省自然科学基金
关键词
癌胚抗原
启动子
基因治疗
TK基因
肺腺癌细胞
carcinoembryonic antigen
herpes simplex virus
thymidine kinase gene
gene therapy
