摘要
根癌农杆菌与来自水稻成熟种子的愈伤组织共培养，将外源基因导入水稻愈伤组织，并获得了转基因植株．通过比较影响根癌农杆菌转化频率的各种因素，表明在转化过程中酚类化合物和单糖的加入使农杆菌的转化频率提高了０．９％～１７．４％．
We successfully introduced foreign genes to calli of rice and got transgenic plants by Agrobacterium tumefaciens cocultured with calli induced from mature seeds of rice.Affer investigating the various factors influencing the transformation frequency of Agrobacterium tumefaciens ,the results indicated that transformation frequency was increased by 0.9% 17.4% supplemented with phenolic compounds and monosaccharide during trans formation step.The dilution way of Agrobacterium tumefaciens played an important role influencing the transformation frequency of Agrobacterium tumefaciens .
出处
《云南大学学报（自然科学版）》
CAS
CSCD
1999年第2期116-119,共4页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省自然科学基金
关键词
水稻
愈伤组织
根癌农杆菌
转化频率
影响因子
Agrobacterium tumefaciens
callus of rice
tansformation frequency
influence factor
