摘要
目的：探讨EB病毒早期抗原（EA）IgG抗体测定在鼻咽癌筛查中的价值。材料和方法：依序列测定方法，用免疫酶染色法对从6257份血清筛查后获得的，IgA／VCA滴度≥1：80的158例血清再进行EBVIgG／EA酶联免疫吸附法（ELISA）测定和IgA／EA抗体免疫酶染色法测定。同时对上述158例血清阳性者进行鼻咽光纤镜和病理检查。根据检查结果比较单项阳性与双项检查阳性血清间鼻咽癌的检出率（阳性预示值）。结果：在这158例阳性血清者中检出鼻咽癌12例。三组阳性血清诊断鼻咽癌的预示值分别是，第一组（IgA／VCA≥1：80）为7．6％（12／158），第2组（IgA／VCZ≥1：80＋IgA／EA≥1：5）为18．2％（8／44），第3组（lgA／VCA≥1：8和lgG／EAOD值≥0．18）为15．9％（11／69）。第一组的阳性预示值最低（P＜0．05），其余两组间无差异P＞005）。第3组检出的11例鼻咽癌包括了第2组中所有的8例鼻咽癌和另外3例IgA／EA阴性鼻咽癌。结论：从序列测定获得的双项阳性血清中筛查到的鼻咽癌比率高于单项lgA／VCA阳性血清。用IgG／EA与IgA／VCA组合测定的双项阳性血清比用IgA／EA与IgA／VCA组合测定的双项阳性血清能查出更多的鼻咽癌，因此该组合更适合于鼻咽癌筛查。
Purpose: To study the value of detection for EBV IgG/EA antibody in screening program for NPC. Material and methods: With the ordinal test mode, the sera of 158 cases (EBV IgA/VCA 1: 80) from 6257 cases screening POpulation were tested again for EBV IgG/EA and EBV IgA/EA antibody by enzyme linked imumosorbent assay (ELISA)and immu－enZymatic method. The 158 seropositive subjects were examined by nasopharyngofi broscope / pathology for N PC.The comparison of the positive predictive value of diagnosis to NPC (NPC－PV) was completed during the three serum groups.Results: 12 NPC were diagnosed in the 158 subjects. The NPC－PV(number of NPC/number of positive sera) in 3 serum ~Ps was: 7. 6% (12/ 158) in group(1 ) (IgA/VCA only ≥ 1: 80), 18. 2% (8/M) in group(2) (lgA/VCA≥ 1: 80 + IgA/EA31: 5), 15. 9% (l I /69) in group(3 ) (IgA/VCA ≥ l: 80 + IgG/EA OD30. 18) respectively. The NPC－PV in coup(l )was lower than other 2 coups (P < 0. 05 ). There was no significant difference of NPC－PV (P > 0. 05) between group(2)and coup(3 ). But, the eleven NPC in coup(3 ) included all NPC in group(2) (8 cases) and other 3 NPC with IgA/EA (－－). ConcluSion: The sera with dual positiveness had higher NPC－PV than the sera with only IgA/VCA3 1: 80. The more NPC would be diagnosed in the sera with IgA/VCA ≥ l: 80 and IgG/EA OD ≥ 0. 18 than in the sera with IgA/VCA ≥1: 80 and IgA/EA ≥1: 5. It is suggested that the ordinal test mode for IgG/EA and IgA/VCA antibody should be taken in screening Program for NPC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第2期190-192,共3页
Chinese Journal of Cancer
基金
广东省卫生厅科研基金!A1997196
关键词
鼻咽肿瘤
EB病毒
筛查
IgG-EA抗体
Nasopharyngeal carcinoma
population screening
EB virus
Early antigen
