摘要
目的探索腺病毒载体用作眼科基因治疗以及视网膜移植物标记的可行性。方法用腺病毒介导ＬａｃＺ基因转导视网膜色素上皮（ＲＰＥ）细胞；ＭＴＴ法测定毒性影响。结果腺病毒滴度为４．６×１０６时可使ＲＰＥ细胞１００％感染；ＬａｃＺ基因表达阳性细胞整个呈蓝色；腺病毒转导与未转导的ＲＰＥ细胞间ＯＤ５７０值无显著性差异。结论就ＲＰＥ细胞而言，腺病毒载体具有很高的转导效率、安全可靠，可用于它的基因治疗。
ObjectiveTo explore the feasibility of application of adenovirus vector to gene therapy in the field of ophthalmology and label of retinal graft.MethodsWe used a recombinant replication deficient adenovirus,AdE1CMV LacZ,which contained the wild type β galactosidase (β gal) gene.Adenovirus mediated LacZ gene was transduced into retinal pigment epithelial(RPE) cells by AdE1CMV LacZ;MTT assay was used to evaluate the cytotoxic effects of AdE1CMV LacZ on RPE cells.ResultsThe transduction efficiency reached 100% at 4.6×10 6 pfu/ml;Positive cells of expression of LacZ gene exhibited whole cell blue pattern after x gal staining.There were not significant differences between the OD 570 of transduced and untransduced RPE cells.ConclusionsAdenovirus vector is a safe and efficient vector.It is useful in targeting RPE cells for the delivery of genes whose products may convey therapeutic benefit to diseases related to RPE cells.Its another potential application would be to transduce marker genes into retinal graft.
出处
《眼科研究》
CSCD
1999年第2期101-103,共3页
Chinese Ophthalmic Research
基金
国家自然科学基金
