摘要
利用8个微卫星DNA位点分析南海海域5个军曹鱼全人工繁育群体(HN1、HN2、ZJ、FJ和LS)子代的遗传多样性特征和群体间遗传分化。结果显示,军曹鱼养殖群体与天然群体的遗传结构特征基本一致:1)平均有效等位基因数为3.910±0.440,观测杂合度为0.595±0.049,分子方差分析(AMOVA)表明,个体内分化占主导(46%),军曹鱼养殖群体整体遗传多样性较高;2)群体间基因流明显(N_m=2.5959,F_(st)=0.0878),整体分化程度较低。各养殖群体表现出不同于天然大群体的特征:1)绝大部分位点均明显偏离哈温平衡,杂合子缺失或过剩现象普遍存在;2)聚类和群体分配分析等表明HN2与另四个养殖群体(HN1、ZJ、FJ和LS)分化明显。
Eight microsatellite loci were employed to elucidate genetic diversity of five cobia（Rachycentron canadum） cultured stocks in the South China Sea（named population HN1,HN2,ZJ,FJ and LS）.The result showed as follows：the overall genetic characteristics of the total cultured population were consistent with the wild（the mean allele number was 3.910±0.440,and mean observed heterozygosity was 0.595±0.049,and AMOVA revealed the proportion of genetic variation within individual was 46%）,and the genetic diversity of cultured populations was moderate with minor decrease.While the obvious deviation from Hardy-Weinberg equilibrium and heterozygote deficiency or excess were observed in each cultured population because of their small founding broodstocks.Although the overall gene flow was obvious for gene flow value Nm was 2.595 9 and the fixation index Fst was 0.087 8,two distinct groups of strains（HN2 and the others） were shown by a population assignment analysis and a phylogenic tree.
出处
《广东海洋大学学报》
CAS
2010年第3期16-21,共6页
Journal of Guangdong Ocean University
基金
"十一五"国家科技支撑计划项目(2007BAD29B03)
关键词
军曹鱼
养殖群体
微卫星DNA
遗传多样性
cobia（Rachycentron canadum）
cultured population
microsatellite DNA
genetic diversity
