摘要
目的观察排铅食品对铅中毒小鼠矿物质的影响。方法选用90只18～22 g昆明种雄性小鼠,随机分为阴性对照组、醋酸铅模型组、阳性药物组和受试物低、中、高剂量组。除阴性对照组自由饮用含12.50μL/L醋酸去离子水外,其余各组均自由饮用1.00 g/L醋酸铅水溶液染毒。30 d后,剂量组按人体推荐摄入量0.60 g/(kg.d)的5,10和20倍经口灌胃受试物,阳性药物组灌胃二巯基丁二酸溶液,醋酸铅模型组和阴性对照组灌胃去离子水。1月后测全血、肝、股骨、肾和脑中矿物质的含量。结果与醋酸铅模型组相比,受试物各剂量组血、肝和脑铅水平显著降低;中、高剂量组骨、肾铅水平显著降低;各剂量组全血钙、铜、肾铜、脑钙水平显著降低,全血铁、骨钙、脑铁水平显著升高;中、高剂量组血锌、骨锌、肾铁水平显著升高,肝锌、肾锌水平显著降低;高剂量组脑铜水平显著降低,肝铁、脑锌水平显著升高。结论排铅食品有明显改善铅中毒小鼠矿物质代谢紊乱的作用。
Objective To observe the effect of lead-removing food on mineral matter in lead poisoned mice.Methods Ninety Kunming male mice（18 ~ 22 g） were randomly divided into negative control group,lead acetate model group,positive medicine group,low dose group,medium dose group and high dose group.Except the negative control group with free drinking deionized water containing 12.50 μL/L of acetic acid,other groups were free to drink 1.00 g/L lead acetate aqueous solution for poisoning.30 days later,the tested compound was given by oral gavage to different dose groups with 5,10 20-fold of the recommended human intake of 0.60 g/（kg？d）,respectively,and dimercaptosuccinic acid solution was given to positive medicine group and deionized water was given to lead acetate model group and negative control group.A month later,the content of mineral matter was determined for whole blood,liver,femur,kidney and brain.Results Compared with lead acetate model group,the levels of lead in blood,liver and brain in the tested dose groups were signif icantly decreased,the levels of lead in femur and kidney in the medium and high dose groups were signif icantly decreased.And the levels of calcium in whole blood and brain and the levels of copper in whole blood and kidney were signif icantly decreased,but the levels of iron in whole blood and brain and the level of calcium in femur were signifi cantly increased for low,medium and high dose groups.Besides,compared with lead acetate model group,the levels of zinc in whole blood and bone and the level of iron in kidney were signifi cantly increased,but the levels of zinc in liver and kidney were signifi cantly decreased for medium and high dose groups.And the levels of copper in brain were signif icantly decreased,while the levels of iron in liver and zinc in brain were significantly increased for high dose group.Conclusion Lead-removing food can significantly improve mineral metabolic disorders in mice with lead poisoning..
出处
《食品与药品》
CAS
2010年第7期247-251,共5页
Food and Drug
关键词
排铅食品
铅中毒
矿物质
小鼠
lead-removing food
lead poisoning
mineral
mice
