摘要
目的研究心力衰竭患者细胞凋亡抑制因子（Ａｐｏ１／Ｆａｓ）水平变化，评估衰竭心肌细胞凋亡状态。方法采取链霉亲和素生物素ＥＬＩＳＡ法测定６０例心力衰竭患者和５２例正常对照组血清Ａｐｏ１／Ｆａｓ，白细胞介素（ＩＬ）６和肿瘤坏死因子α（ＴＮＦα）水平，并测定心力衰竭患者左室射血分数（ＥＦ），了解ＥＦ变化与Ａｐｏ１／Ｆａｓ间的关系。结果心功能Ⅲ、Ⅳ级患者血清Ａｐｏ１／Ｆａｓ，ＴＮＦα显著高于对照组及心功能Ⅰ、Ⅱ级患者（Ｐ值＜０．０１）；所有心功能不全患者血清ＩＬ６水平均明显高于对照组（Ｐ值＜００５和０．０１），且心功能Ⅲ、Ⅳ级患者高于心功能Ⅰ、Ⅱ级患者（Ｐ值＜０．０５）；ＥＦ＞５５％组患者血清Ａｐｏ１／Ｆａｓ水平显著高于ＥＦ＞５５％组患者（Ｐ值＜０．０１）。结论心力衰竭患者血清Ａｐｏ１／Ｆａｓ反映了衰竭心肌细胞凋亡状态。ＩＬ６和ＴＮＦα则在心肌细胞凋亡中起重要的免疫调控作用。
Objective To study the changes of serum level of Apo 1/Fas in patients with congestive heart failure (CHF) and evaluate apoptosis of failing myocardial cells. Methods Strepavidin Biotin ELISA was used to determine serum level of Apo 1/Fas, interleukin 6(IL 6) and tumor necrosis factor (TNFα) in 60 patients with CHF. Cardiac ejection fraction (EF)of the patients were measured by acusson 128XP/10 echocardiagraph. Results Serum levels of Apo 1/Fas and TNFα in class Ⅲ and Ⅳ patients with CHF were significantly higher than those in class Ⅰand Ⅱ( P <0.01). Serum levels of IL 6 in all the patients were obviously higher than those in controls ( P <0.05 and 0.01) and the levels in class Ⅲ and Ⅳ patients were significantly higher than those in class Ⅰ and Ⅱ ( P <0.05). Serum levels of Apo 1/Fas in patients with EF<55 percent were higher than in those with EF>55 percent ( P <0.05). Conclusion Serum level of Apo 1/Fas in patients with CHF reflects a state of apoptosis in failing myocardial cells. IL 6 and TNFα have important effects on immune regulation of myocardial cell apoptosis.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1999年第3期168-170,共3页
Chinese Journal of Internal Medicine
