摘要
探讨硫酸镁对大鼠颅脑损伤后脑线粒体ＡＴＰ酶、超氧化物歧化酶（ＳＯＤ）的活性及线粒体Ｃａ２＋、Ｍｇ２＋及ＭＤＡ含量的影响。方法对自由落体致颅脑损伤的ＳＤ大鼠在致伤同时腹腔注射硫酸镁治疗，伤后２４小时测定线粒体Ｎａ＋－Ｋ＋ＡＴＰ酶、Ｃａ２＋－ＡＴＰ酶、Ｍｇ２＋－ＡＴＰ酶、ＳＯＤ的活性及线粒体Ｃａ２＋、Ｍｇ２＋及ＭＤＡ含量。结果颅脑损伤后大鼠脑线粒体Ｎａ２＋－Ｋ＋ＡＴＰ酶，Ｃａ２＋－ＡＴＰ酶，Ｍｇ２＋－ＡＴＰ酶的活性均明显下降（Ｐ＜０．０５），线粒体Ｃａ２＋、ＭＤＡ含量增加，Ｍｇ２＋含量及ＳＯＤ活性明显降低（Ｐ＜０．０５）。与创伤组相比，硫酸镁治疗组上述指标均有明显改善（Ｐ＜０．０５）。结论硫酸镁可以改善损伤后脑线粒体酶活性并减轻线粒体钙超载。
Objective To investigate the therapeutic effects of magnesium sulfate on the activities of ATPase, SOD and the content of MDA, Ca2+, Mg2+ in brain mitochondria after brain trauma inrats. Method The magnesium sulfate was administered intraperitoneally immediately following braintrauma resulted from free-falling. The mitochondria were isolated from rat brain at 24 h after traumawhile the activities of Na+--K+-ATPase, Ca2＋-ATPase, Mg2+-ATPase, SOD and the content of MDA,Ca2+,Mg2+ in brain mitochondria were measured. Result The activities of Na+-KATPase, Ca2+-AT-Pase, Mg2+-ATPase, SOD were reduced significantly but the level of MDA and Ca2+ elevated markedlyafter brain trauma (P<0.05). However, these changes were suppressed in treatment group. Conclusion Treatment with MgSO4 in early stage can improve the activities of mitochondrial Na+-K+ AT Pase,Ca2+-ATPase, Mg2+-ATPase, SOD and inhibit calcium overload in mitochondria after brain inJury.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1999年第2期164-165,共2页
Chinese Journal of Experimental Surgery
