摘要
构建人肝癌组织特异性的基因治疗载体并探讨其临床意义。方法利用重组ＤＮＡ技术将ＨＳＶ－ＴＫ基因插入到含有及不含有α－ＦＰ启动子的ＥＢ病毒表达载体ＰＥＢＡＦ５．１、ｐＤＲ２中，并通过限制性内切酶分析鉴定重组质粒。结果ＴＫ基因成功地克隆入ＰＥＢＡＦ５．１及ｐＤＲ２中。结论含有α－ＦＰ启动子的ＥＢ病毒表达载体是原发性肝癌基因治疗中新型、理想的候选载体之一。
Objective To construct human hepatocellular carcinoma tissue-specific gene therapyvectors, and study its clinical implication. Method HSV-TK gene was inserted into EBV-based vectorpDR2 and pEBAF5.1 containing α-FP promoter and enhancer by using recombinant DNA technique.The recombinant plasmids were analyzed and identified by restriction enzyme. Result 1. 79 kb TK genefragments were separately successfully cloned into PDR2 and PEBAF5.1 by restriction enzyme analysis.Conclusion EBV-based vector harboring α-FP promoter and enhancer is a novel ideal candidate vectorfor hepatocellular carcinoma gene therapy.
出处
《中华实验外科杂志》
CSCD
北大核心
1999年第2期153-154,共2页
Chinese Journal of Experimental Surgery
基金
863国家高技术发展计划
关键词
肝肿瘤
EB病毒
载体
HSV-TK基因
Hepatocellular carcinoma
EBV-based vector
HSV-TK gene
α-FP promoter
