摘要
目的：探讨成纤维细胞是否为晚期糖基化终产物修饰的β２微球蛋白（ＡＧＥ－β２Ｍ）生物学效应的靶细胞。方法：放射性配体结合实验；［３Ｈ］－ＴｄＲ试验和直接密度计数。结果：［１２５］Ｉ－ＡＧＥ－β２Ｍ能以特异、剂量依赖的方式与人成纤维细胞（ＨＳＦ）结合，这一过程可被抗ＲＡＧＥＩｇＧ所抑制。ＡＧＥ－β２Ｍ能刺激ＨＳＦｓ的增殖，该反应可被抗ＲＡＧＥＩｇＧ、但不被抗ＥＧＦ中和抗体所抑制。结论：ＡＧＥ－β２Ｍ可能通过与成纤维细胞ＲＡＧＥ的相互作用参与透析相关性淀粉样变的发生。
AIM:To test the hypothesis that fibroblast may be a target for the biological action of β 2-microglobulin modified with advanced glycation end products(AGE-β 2M).METHODS:Radioligand-binding study;-TdR and direct density quantification.RESULTS: 125 I-AGE-β 2M bound to a human skin fibroblast cell line(HSF) in a specific,dose-dependent manner,a process inhibited in the presence of anti-RAGE IgG.HSFs exposed to AGE-β 2M showed upregulation of proliferation which could be inhibited by anti-RAGE IgG,but not by anti-epidermal growth factor neutralizing antibody.CONCLUSION:AGE-β 2M may play a role in the pathogenesis of dialysis-related amyloidosis via a pathway involving a fibroblast receptor for AGE.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第3期206-209,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
