摘要
研究树突状细胞（ＤＣ）体外诱导的细胞免疫抑制裸鼠移植瘤生长作用及其机理。方法：联合应用粒／巨噬细胞集落刺激因子ＫＭ－ＣＳＦ）及白介素－４（ＩＬ－４）直接从肝癌患者外周血中培养出ＤＣ，以人肝癌细胞系ＨｅｐＧ２肿瘤细胞的肿瘤抗原粗提物刺激ＤＣ，ＤＣ激活同源的Ｔ淋巴细胞，建立裸鼠人肝癌细胞系ＨｅｐＧ２移植瘤模型，以被激活的Ｔ淋巴细胞治疗裸鼠ＨｅｐＧ２移植瘤并观察治疗效果，检测移植瘤标本肿瘤细胞凋亡情况。结果：ＤＣ诱导的Ｔ细胞免疫能够诱导裸鼠人肝癌细胞系ＨｅｐＧ２移植瘤肿瘤细胞大量凋亡从而抑制裸鼠ＨｅｐＧ２移植瘤生长。结论：经肿瘤抗原激活的ＤＣ作为一新概念上的抗肿瘤疫苗有可能在肿瘤的治疗中发挥重要作用。
Objective: To study the mechanism of antitumor immune response induced by dendriticcells (DC) in the inhibition of growth of implanted tumor in nude mice by way of inducing tumor cell apop-tosis. Methods: Isolated and purified DC derived from hepatocellular cancer (HCC) patients with granulo-cyte/macrophage colony stimulating factor and interieukin 4, extracted tumor - associated antigen(TAA) from human hepatocellular cancer cell line HepG2 tumor cells, Stimulated T lymphocytes with DCpulsed by TAA to produce CTL fcytotoxic T lymphocyte) , Implanted the CTL to inhibit the growth of im-planted tumor in nude mice, Evaluated the apoptosis of tumor cells. Results: The DC from HCC patientspulsed with TAA from HepG2 tumor cells could stimulate T lymphocyte immune response inhibiting thegrowth of implanted tumor in nude mice by way of inducing tumor cell apoptosis. Conclusion: As a newconcept anti - tumor vaccine of DC pulsed by TAA may play an important role in therapy of tumor.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1999年第3期222-224,共3页
Chinese Journal of Clinical Oncology
关键词
树突状细胞
肿瘤疫苗
肝肿瘤
细胞凋亡
Dendrite cells,Tumor immunity,Anti - tumor vaccine,Apoptosis
