摘要
目的探讨不育男性精子顶体内透明质酸酶活性与精液有关参数的关系,以期为临床男性不孕不育的病因分析、诊断、治疗及精子的功能检查提供理论及实验依据。方法收集81例精液标本,其中正常生育对照组为20例,不育男性61例,以精子质量分析仪对每份标本进行计算机辅助精液分析(CASA)。并利用改良明胶底物膜法检测精子顶体内透明质酸酶活性。结果①不育组与对照组相关动态参数的分析结果:VCL、VSL、VAP、LIN、WOB的数据不育组与对照组具有统计学意义,表明不育组精子综合质量与对照组间存在差异;②精子运动活力的参数即精子的VCL、VSL、VAP以及ALH等精子参数与精子顶体内透明质酸酶活性有显著相关性,与LIN、STR、WOB、BCF及STR无统计学意义。结论①不育组与对照组相关动态参数VCL、VSL、VAP、LIN、WOB数据具有统计学意义,精子综合质量与对照组间存在差异;②精子参数VCL、VSL、VAP及ALH与精子顶体内透明质酸酶活性有显著相关性。
Objective To study the relationship between sperm acrosin hyaluronidase activity in infertilitle men and conventional semen parameters in order to provide theoretical and experimental basis for analysis of cause, diagnosis, treatment of clinical male infertile, and for sperm function tests. Methods Eighty - one semen samples were collected. Twenty cases were in normal control group, and other cases were infertile men （ excluding the infertility caused by women ）. Conventional clinical examination of each semen specimen was made by using sperm quality analysis instrument and computerassisted sperm analysis （CASA）. Sperm hyaluronidase activity was detected by using the method of modified gelatin membrane. Results （1）There was significant difference in the related dynamic parameters of VCL, VSL, LIN and WOB between the infertile men and control persons which indicated that sperm quality was unnormal. （2）Sperm motility parameters （VCL,VSL, VAP 以及 ALH） were related significantly with sperm hyaluronidase activity. There was no statistical significance in LN,STR,WOB,BCF and STR between the two groups. Conclusion （1）The infertile group and control group showed statistical significanc in relevant dynamic parameters such as VCL, VSL, VAP, LN and WOB. The integrated quality of the sperm in infertile group is different from that of control group. （2）There is a significant correlation between the sperm parameters （VCL, VSL, VAP, ALH） with sperm acrosin hyaluronidase activity.
出处
《医学研究杂志》
2010年第8期59-62,F0003,共5页
Journal of Medical Research
基金
贵州省省长专项基金(黔省专合字200746号)
贵州省科技厅攻关项目(黔科合S字20071030)
黔南州科技项目(黔南科合20085)
关键词
精子
透明质酸酶
精液参数
关系
Sperm
Hyaluronidase
Semen
Parameters
Relation.
