摘要
目的探讨整合素连接激酶(ILK)在病毒性心肌炎(VMC)的发生、发展过程中的作用,及ILK和转化生长因子(TGF)-β1在心肌纤维化形成过程中的作用。方法应用柯萨奇病毒B3(CVB3)感染Balb/c小鼠,建立VMC小鼠模型。实验分为正常对照组(n=30)和病毒组(n=40)。在CVB3感染后第7、14、28、56天应用免疫组织化学方法检测各实验组ILK和TGF-β1的表达,对心肌胶原纤维进行Masson染色和测定血清CK-MB水平,同时分析各实验组不同时间点心肌组织中ILK和TGF-β1阳性表达与心肌胶原纤维Masson染色阳性面积及与血清CK-MB水平的相关性。结果正常对照组无或仅有极少量的ILK和TGF-β1阳性表达,病毒组在病毒感染后各时间点ILK和TGF-β1阳性表达面积均明显增加,与对照组比较差异有统计学意义(P<0.01),且ILK和TGF-β1阳性表达面积存在直线相关性(r=0.970,P<0.01);病毒组小鼠于病毒感染后第14天开始Masson染色阳性面积明显增多(P<0.05);且病毒组小鼠心肌组织中ILK阳性表达面积与心肌胶原纤维Masson染色阳性面积存在直线相关性(r=0.996,P<0.01);心肌组织中ILK的阳性表达面积与血清CK-MB无相关性(r=-0.624,P>0.05)。结论 ILK的异常表达在VMC的发生、发展中起重要作用,在VMC发病进程中ILK参与了心肌纤维化的形成过程。
Objective To explore the role of integrin-linked kinase （ILK） in the process of the occurrence and development of viral myocarditis （VMC） and the effect of ILK, TGF-β1 in myocardial fibrosis. Methods Balb/c mice was infected by coxsackievirus B3 （CVB3） to establish VMC model. The mice were divided into control group （n = 30） and virus group （n = 40）. At infected 7, 14, 28, and 56 days, the levels of ILK and TGF-β1 were detected by streptavidin peroxidase conjugate. The area of positive Masson stained myocardium collagen fibers was measured, and the level of serum creatine phosphokinase-MB （CK-MB） was determined. At the same time, the relationship between the expression of ILK and TGF-β1, the positive area of myocardium collagen fibers, and the serum level of CK-MB were analyzed. Results There were no or little expression of ILK and TGF-β1 in control group. Meanwhile, in virus group, the expressions of ILK and TGF-β1 were much higher than that in control group （P 〈 0.01）. The expression of ILK and TGF-β1 was positively linear correlated （r = 0.970, P 〈 0.01）. The positive Masson stain area of myocardium collagen fibers in virus group was significantly increased than that in control group at 14th day infected CVB3 （P 〈 0.05）. The expression of ILK was positively correlated with the positive Masson stain area of myocardium （r = 0.996, P 〈 0.01）, but it was not correlated with the level of serum CK-MB （r = -0.624, P 〉 0.05）. Conclusions The abnormal expression of ILK is related with the occurrence and development of VMC, and involved in the process of myocardial fibrosis caused by VMC.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2010年第7期675-678,691,共5页
Journal of Clinical Pediatrics
