摘要
目的:探讨BNIP3基因对人宫颈癌的放射增敏效应的影响。方法:将6×10~6HeLa细胞皮下接种到裸鼠的背部,并通过尾静脉将质粒DNA注射到裸鼠体内,建立动物肿瘤模型。肿瘤体积按A×B^2×0.5计算。用免疫组化的方法来检测肿瘤组织中Bnip3蛋白的表达,TUNEL法检测肿瘤细胞凋亡。结果:免疫组化测得,pDsRed-BNIP3处理组的肿瘤细胞中Bnip3蛋白的表达明显较其他组高。这个凋亡调节因子在体内能明显地增加肿瘤细胞的凋亡数(P<0.01)。肿瘤生长曲线也显示BNIP3基因可以增强放射治疗的抗肿瘤效应。结论:重组质粒pDsRed-BNIP3在体内对宫颈癌HeLa细胞移植瘤有放射增敏效应。
Objective： To investigate the effects of BNIP3 gene on the radiosensitivity of human cervical cancer. Methods： 6 × 10^6 HeLa cells was subcutaneously inoculated into the dorsal flank of nude mice and plasmid DNA was injected into mice via tail vein to establish the animal tumor model. Tumor volume was calculated using the equation A × B^2×0.5. Immunohistochemistry was used to detect the expression of Bnip3 in tumor tissues, and TUNEL assay was performed to determine apoptosis of tumor cells in vivo. Results： Immunohistochemistry revealed that the expression of Bnip3 protein in tumor cells was increased when treated with pDsRed- BNIP3 . This apoptosis regulator significantly increased cell apoptosis （P 〈 0. 01 ） in vivo. Tumor growth curve showed that the antitumor effect of radiotherapy was enhanced by BNIP3. Conclusion ： Recombinant pDsRed-BNIP3 plasmid can increase the radiosensitization of human cervical cancer HeLa xenografts in vivo. BNIP3 may play a role in enhancing the efficiency of radiotherapy.
出处
《肿瘤预防与治疗》
2010年第4期269-273,共5页
Journal of Cancer Control And Treatment
