摘要
目的：以原代培养的大鼠胎鼠皮质神经元低氧低糖再复氧为模型，研究丁基苯酞对神经细胞凋亡的抑制作用。方法：用流式细胞术检测ＤＮＡ含量及凋亡细胞百分率，透射电镜观察细胞形态学变化，ＤＮＡ琼脂糖凝胶电泳和原位末段标记（ＴＵＮＥＬ）检测ＤＮＡ断裂。结果：丁基苯酞能减轻细胞核形态的改变，减少ＤＮＡ断裂和阳性细胞数，使低氧低糖诱导的神经细胞凋亡百分率明显下降，凋亡峰显著降低。结论：丁基苯酞对低氧低糖诱导的大鼠皮质神经细胞凋亡有抑制作用。
AIM: The effect of dl 3 n butylphthalide on cerebrocortical neuronal apoptosis induced by hypoxia/hypoglycemia was investigated. METHODS: The DNA content and percentage of apoptosis were measured by flow cytometry; Morphological changes were observed with light microscope and electron microscopy; DNA fragmentation was analyzed by agarose gel electrophoresis and terminal dUTP nick end labeling(TUNEL) method. RESULTS: The result showed that hypoxia/hypoglycemia(12 h) and reoxygenation(12 h) medium induced apoptosis in primary cultured cortical neurons. dl 3 n Butylphthalide(10 μmol·L -1 ) was found to decrease the percentage of neuronal apoptosis, prevent DNA fragmentation, ameliorate morphological changes of cortical neurons and reduce the numbers of positive cells. CONCLUSION: These findings indicate that dl 3 n butylphthalide prevented hypoxia/hypoglycemia induced apoptosis of rat cortical neurons. These results further revealed the mechanisms of dl NBP in reducing the volume of the cerebral infarct after middle cerebral artery occlusion in rats.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第3期176-180,共5页
Acta Pharmaceutica Sinica
关键词
丁基苯酞
低氧
低糖
皮质细胞凋亡
脑缺血
dl 3 n butylphthalide
hypoxia/hypoglycemia
neurons
apoptosis
