摘要
用新底物２－氯－４－硝基苯－α－半乳糖－麦芽糖苷（Ｇａｌ－Ｇ２－α－ＣＮＰ）直接测定α－淀粉酶，不需要辅助酶，延滞时间短（＜１５ｓ），线性范围宽（可达２２００Ｕ／Ｌ），试剂稳定性好，不使用ＫＳＣＮ、ＮａＮ３等激活剂，不受内源性葡萄糖苷酶的干扰，与ＥＰＳ法对比相关良好：Ｙ＝１．２７Ｘ＋０．５０，ｒ＝０．９９９０。
Studied a method for direct measuring α amylase activity with a new substrate of 2 choro 4 nitrophenyl α galactosylmaltoside.Without the need for auxiliary enzymes,the lag phase of this test was <15 s,the linear range up to 2 200 U/L and the reagent was stable.In this method,KSCN and NaN 3 not used as activators and interference of endogenous α Glucosidase was not found.Compare with EPS method( X )yield a regression of Y=1 27 X+0 50,r =0 9990.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1999年第2期86-88,共3页
Chinese Journal of Clinical Laboratory Science
