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缺氧诱导因子-1α与缺氧状态下胃癌细胞侵袭的相关性 被引量：6

Relationship of Hypoxia-inducible Factor-1α and Invasion of Gastric Cancer Cells in Hypoxia
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摘要 目的探讨缺氧状态下及缺氧诱导因子-1α(Hypoxia-inducible factor-1α,HIF-1α)RNA干扰后,HIF-1α的表达及其与胃癌细胞黏附、游走、侵袭能力的相关性。方法将HIF-1α shRNA真核表达质粒pGPU6/GFP/Neo-HIF-1α-2484转染胃癌细胞SGC-7901,构建靶向干扰HIF-1α表达的SGC-7901稳定转染细胞株;采用Western blot法检测缺氧及HIF-1αRNA干扰对SGC-7901细胞HIF-1α表达的影响;MTT法检测缺氧及HIF-1αRNA干扰对SGC-7901细胞黏附能力的影响;采用Boyden Chamber膜侵袭系统,观察缺氧及HIF-1αRNA干扰对SGC-7901细胞游走及侵袭能力的影响。结果 SGC-7901细胞在缺氧培养4、8和16h时,HIF-1α均呈阳性表达,并随时间的延长而显著增加;细胞粘贴率、游走和侵袭穿膜细胞数分别随时间延长而显著增加,与HIF-1α表达呈显著正相关。HIF-1αRNA干扰后,缺氧SGC-7901细胞HIF-1α表达显著下降,细胞粘贴率、游走和侵袭穿膜细胞数均显著下降。结论 HIF-1α的过度表达导致胃癌细胞黏附、游走及侵袭能力增强,可能是胃癌局部侵袭和远处转移的重要原因之一。 Objective To investigate the expression of hypoxia-inducible factor-1α（HIF-1α）and its relationship to the adhesion,migration and invasion abilities of human gastric cancer cells in hypoxia and HIF-1α RNA interference（RNAi）.Methods Gastric cancer SGC-7901 cells were transfected with eukaryotic expression vector pGPU6 /GFP /Neo-HIF-1α-2484 to construct a stably transfected SGC-7901 clones of HIF-1α RNA.The effects of hypoxia and HIF-1α RNAi on expression of HIF-1α in SGC-7901 were determined by Western blot,on adhesion ability of SGC-7901 cells by MTT method,and on migration and invasion abilities by Boyden Chamber membrane invasion system.Results HIF-1α was expressed in SGC-7901 cells 4,8 and 16 h after culture in hypoxia,and its expression level increased significantly with the increasing hours for culture.The adhesion rate as well as the counts of migrated and transmembrane invaded SGC-7901 cells increased significantly with the increasing hours for culture,and showed significantly positive relationship to HIF-1α expression.After HIF-1α RNAi,the expression level of HIF-1α,adhesion rate as well as the counts of migrated and transmembrane invaded hypoxic SGC-7901 cells in hypoxia decreased significantly.Conclusion The overexpression of HIF-1α enhanced the adhesion,migration and invasion abilities of gastric cancer cells,which might be one of the important reasons for local invasion and distant metastasis of gastric cancer.
作者 邓婷 张俊文
机构地区 重庆医科大学附属第一医院消化内科
出处 《中国生物制品学杂志》 CAS CSCD 2010年第7期696-699,共4页 Chinese Journal of Biologicals
基金 重庆医科大学2005年创新基金(cx200504)
关键词 缺氧诱导因子1 Α亚基 胃肿瘤 RNA干扰 细胞黏附 游走 肿瘤侵袭 Hypoxia-induciblefactor-1 subunitα（HIF-1α） Gastrictumor RNAinterference Celladhesion Migration Tumorinvasion
分类号 R735.2 [医药卫生—肿瘤] R364.4 [医药卫生—病理学]
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共引文献23

同被引文献49

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  • 3张俊文,王丕龙.缺氧时缺氧诱导因子-1α在胃癌细胞的表达及其同细胞增殖凋亡的关系[J].肿瘤防治研究,2006,33(9):635-637. 被引量：5
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中国生物制品学杂志
2010年 第7期

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