摘要
目的通过对椎间盘细胞的培养，观察细胞的演变，以便更深入地研究椎间盘退变的细胞因素。方法取３例人体胚胎、１例正常成人的腰椎间盘，在ＨＡＭＦ１２加质量分数为１０％的灭活胎牛血清培养液中，建立了体外人椎间盘细胞培养模型，并对细胞进行了冻存复苏、光镜及透射电镜观察。结果（１）原代、次代的椎间盘细胞的生物学性状最接近体内的细胞。随传代次数的增加，脊索细胞及软骨细胞表现出反分化的特性。（２）解冻复苏细胞的形态及代谢能力与冻存前基本相同。（３）椎间盘细胞内细胞器的结构变化与其生物学活性的变化相一致。结论人体腰椎间盘细胞体外培养的成功。
Objective To observe the continuous changes of disc cells, and explore the degeneration influenced by the cell effects.Methods Three discs were obtained from three human embryoes and one was from a healthy adult. They were dissected and were digested with trypsin and collagenase. The isolated cells were cultured in HAM F-12 medium and 10% fetal bovine serum. The cultured cells were refrigerated and revived for observation under light microscope and TEM. Results The biological characteristics of the primary and the second generation of the disc cells were similar to those of the cells in vivo. After subculture, notochordal cells and chondrocytes were shown the characteristics of dedifferention. The metabolism of the revived cells after refrigeration was normal. The structures of the organellae in disc cells varied with the changes of cell biological characteristics.Conclusion This successful model is useful for the study of biology and pharmacology of human disc cells.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第2期109-111,共3页
National Medical Journal of China
关键词
椎间盘
细胞
培养
形态学
Intervertebral disc Cells,cultured
