摘要
目的观察缺氧性肺动脉高压（ＨＰＨ）大鼠离体肺动脉环对蛋白激酶Ｃ（ＰＫＣ）激活剂豆蔻酸佛波酰乙酯（ＰＭＡ）的反应性变化。方法取缺氧２周并已经形成肺动脉高压的大鼠和正常对照组大鼠肺动脉环，观察在离体情况下对０．５μｍｏｌ／ＬＰＭＡ的最大张力反应（Ｐ１）及达到二分之一最大张力（Ｐ１／２）所需的时间（ｔ１／２），并描绘两组肺动脉环对０．０１～１０．０μｍｏｌ／ＬＰＭＡ的浓度反应曲线，根据曲线推算ＥＣ５０ＫＣｌ（即对ＰＭＡ的反应值达到８０ｍｍｏｌ／ＬＫＣｌ引起的最大反应值的一半时所对应的ＰＭＡ浓度）。结果缺氧组（６只）Ｐ１［以８０ｍｍｏｌ／ＬＫＣｌ反应的最大值为Ｐ０，Ｐ１以其相当于Ｐ０的百分数（Ｐ０％）表示］为（３４．３±２．４）Ｐ０％，与正常组［６只，（２６．２±２．９）Ｐ０％］比较差异有显著性（Ｐ＜０．０５）。缺氧组（６只）ｔ１／２为（２７±７）ｍｉｎ，与正常组［６只，（３２±５）ｍｉｎ］比较差异有显著性（Ｐ＜０．０５）。缺氧组（１２只）ＥＣ５０ＫＣｌ为（０．７±０．１）μｍｏｌ／Ｌ，与正常组［１２只，（６．０±０．２）μｍｏｌ／Ｌ］比较差异有显著性（Ｐ＜０．０５）。结论ＨＰＨ大鼠离体肺动脉环对ＰＭＡ的反应性显?
Objective To observe the reactivity of isolated pulmonary artery rings from rats with chronic hypoxic pulmonary hypertension (HPH) to protein kinase C (PKC) activator. Methods Pulmonary artery rings from adult rats exposed to normobaric hypoxia for two weeks and normal rats were isolated for measurement of isometric contractions to PMA, the specific activator of PKC. Responses were examined as follows:(1) The maximal response (P 1) to 0.5 μmol/L PMA and the time required to achieve a half maximal response to 0.5 μmol/L PMA (t 1/2 );(2)Dose respose curve in response to PMA(0.01～10.0 μmol/L) and the dose of PMA that produced a contractile response as 50% of the maximal response(P 0) to 80 mmol/L KCl (EC 50 KCl). Results P 1 of hypoxic group (34.3±2.4)P 0% was greater than that of normal group [(26.2±2.9)P 0% , n =6, P < 0.05)], t 1/2 and EC 50 KCl of hypoxic group [(27±7)min, and (0.7±0.1)μmol/L] were smaller than those of normal group [(32±5)min, n =6, P <0.05, and (6.0±0.2)μmol/L, n =12, P <0.05]. Conclusions The reactivity of pulmonary artery rings from hypoxic rats to PMA was enhanced, which indicates that protein kinase C may have an important role in the developement of HPH.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1999年第2期92-94,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家自然科学基金
国家教委留学回国人员科研启动基金
