摘要
本文首次报道了应用鸭乙型肝炎病毒(DHBV)加大肠杆菌内毒素诱导方法,建立了鸭急性肝坏死动物模型。结果发现:先肝内注射大肠杆菌内毒素,24h后再静脉内激发性注射DHBV者,易造成一叶或二叶肝坏死。而第一次肝内注射内毒素,24h后静脉内注入生理盐水,或二次均注入生理盐水者均未发现肝坏死病理变化。若第一次肝内注入DHBV,第二次静脉内注入内毒素,病理变化则明显轻于第一种方法,仅出现轻微散在斑点状坏死。
Using duck hepatitis B virus (DHBV) in association with endotoxin, we have established a duck model of acute hepatic necrosis. The procedure consisted of a preparatory injection of E coli endotoxin or DHBV into liver through a catheter placed in the bile duct. and an intravenous injection of DHBV or endotoxin 24 h later for provocation. Liver changes were observed 72 h after the provocative injection. Results showed that DHBV in the duck's liver tissue provoked a Shwartzman reaction with spotty, widely scattered foci of necrosis surrounded by cell infiltration. Endotoxin was found to be capable of inducing or exacerbating acute hepatic failure.
出处
《上海第二医科大学学报》
CSCD
1990年第3期189-193,共5页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
乙肝病毒
肝坏死
鸭
动物模型
duck hepatitis B virus fulminant hepatitis Shwartzman reaction tumour necrotic factor interleukin-1 interferon-γ
