摘要
目的探索深部真菌病组织内病原菌检查的新方法。方法应用生物素卵白素过氧化酶法（ＡＢＣ）研究１７种深部真菌（包括培养中真菌及小鼠实验性感染皮肤及内脏组织内真菌）与１４种凝集素的结合形式。结果１７种真菌的凝集素结合形式稳定。绝大多数真菌的凝集素结合形式呈属间区别，少数亦见种间区别。结论为利用凝集素组织化学鉴定组织内病原菌提供了实验依据。
Objective In order to explore a new method of aetiological diagnosis for deep fungal infection in vivo.Methods The lectin binding patterns of 17 deep fungi (include specimens from cultures and tissue samples from experimentally infected mice)were studied,using 14 different biotinyl lectins and avidin-horseradish peroxidase(ABC).Results Lectin binding patterns of the tested fungi were stable.Most of the tested fungi were distinguishable each other based on the lectin binding patterns of the genus,few were distinguishable of the species.Conclusion The mentioned results offer a experimental basis for differentiating fungi in tissue section using the lectin histochemistry.
出处
《中国皮肤性病学杂志》
CAS
北大核心
1999年第2期73-74,共2页
The Chinese Journal of Dermatovenereology
基金
辽宁省教委基金
