摘要
本文结合活体荧光染料Hydroethidine（HE）和结合流式细胞术，对实验室培养的恶性疟原虫虫血率及其生长周期进行了相对定量研究和分析，以期寻求一种可替代血涂片镜检法的检测方法。结果表明，染料Hydroethidine工作浓度10μg／ml即可获得与50μg／ml相同的效果；Hydroethidine-流式细胞术法与常规血涂片镜检法具有强相关性；此外，该方法可以进一步分析培养恶性疟原虫中早期（环期、滋养体期）和晚期（裂殖体期）的组成和比例。因此，Hydroethidine．流式细胞术法作为一种客观、准确、快速的相对定量分析方法，在疟原虫研究中具有广泛的应用空间，可以替代血涂片镜检法。
Using flow cytometry with vital fluorochrome Hydroethidine （HE）, the working concentration of Hydroethidine was optimized to estimate the parasitemia of Plasmodium falciparum cultured in lab and analyse its life cycle. The results showed that 10 μg/ml HE was used as the optimized concentration, comparable with the routine concentration 50 μg/ml. Meanwhile, when determining parasitemia by flow cytometry and blood smear-microscopy, a strong correlation was found between the two methods. In addition, the trophozoite and schizont stage of parasites could be distinguished on the basis of fluorescence intensity by this method. In conclusion, Hydroethidine-based flow cytometry as a relative quantitative analysis method, can potentially substitute the blood smear-microscopy and be widely used in the study of P. falciparum, since it is objective, accurate and rapid.
出处
《寄生虫与医学昆虫学报》
CAS
2010年第2期77-80,共4页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家“863”项目（No.20060102A2113）
