摘要
采用DNA测序、巢氏PCR和CRS-PCR方法对中国荷斯坦牛、鲁西黄牛、渤海黑牛的趋化因子受体基因1(CXCR1)的单核苷酸多态性(SNPs)进行研究,在第2外显子上发现了4个SNPs,分别为291 bp(C/T)、333 bp(C/T)、337 bp(A/G)和365 bp(C/T),这4个位点在中国荷斯坦牛、鲁西黄牛、渤海黑牛群体中均达到中度多态(0.25<PIC<0.5),优势等位基因分别为C、C、A、C,等位基因频率分别为0.82/0.67/0.65、0.80/0.74/0.71、0.70/0.65/0.82、0.56/0.58/0.56。经χ2适合性检验,渤海黑牛所有位点全部达到Hardy-Weinberg平衡状态;荷斯坦牛在突变位点291 bp(C/T)、333 bp(C/T)和337 bp(A/G)达到Hardy-Weinberg平衡状态;鲁西黄牛仅在365 bp(C/T)位点达到Hardy-Weinberg平衡状态。
The single nucleotide polymorphisms（SNPs） within the chemokine receptor CXCR1 gene in Holstein cattle,Luxi Yellow cattle and Bohai Black cattle were investigated by DNA sequencing technology,nested-PCR and CRS-PCR methods.Sequencing results showed that four SNPs in exon 2 were identified,which they were 291 bp（C/T）,333 bp（C/T）,337 bp（A/G） and 365 bp（C/T）.The value of polymorphism information content indicated（0.25PIC0.5） that all the four SNPs were moderate polymorphism in Holstein cattle,Luxi Yellow cattle and Bohai Black cattle.Furthermore,C,C,A and C predominan allele were identical at 291 bp（C/T）,333 bp（C/T）,337 bp（A/G） and 365 bp（C/T）,with the allelic frequencies 0.82/0.67/0.65,0.80/0.74/0.71,0.70/0.65/0.82,0.56/0.58/0.56 respectivly.Chi-square test indicated all the sites of Bohai Black cattle were in accordance with the Hardy-Weinberg equilibrium,while Holstein cattle meet Hardy-Weinberg equilibrium（P0.05） at 291 bp（C/T）,333 bp（C/T） and 337 bp（A/G）,and the Luxi Yellow cattle meet Hardy-Weinberg equilibrium only at 365 bp（C/T）.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第7期93-98,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家"863"计划项目(2006AA10Z1D9
2007AA10Z1D9)
公益性行业科研专项(nyhyzx07-036-09)
山东省良种工程项目(2007LZ10-05)
关键词
牛
CXCR1基因
单核苷酸多态性
优势等位基因
Bos taurus
CXCR1 gene
single nucleotide polymorphisms（SNPs）
predominan allele
