摘要
为建立一种能够区分口蹄疫疫苗免疫与野毒感染动物的快速检测方法,将纯化的口蹄疫病毒3AB非结构蛋白抗原包被在硝酸纤维素膜上,加入待检血清样品后,利用胶体金标记SPA显色。应用斑点免疫金渗滤技术(DIGFA)和ELISA方法同时对150份猪血清进行检测,结果DIGFA法的阳性率为6%(9/150),ELISA法的阳性率为5.3%(8/150),两者符合率达99.3%。DIGFA操作简单,耗时短,结果易于判断,且与口蹄疫免疫猪血清、猪细小病毒、猪瘟、猪繁殖与呼吸综合征和猪伪狂犬病病毒阳性血清不发生交叉反应。结果表明,该法适用于生猪和牛等口蹄疫病毒3AB抗体的快速检测。
In order to establish a rapid detection method to differentiate foot-and-mouth disease virus（FMDV）vaccinated animals from infected animals,the purified NSP-3AB of FMDV was coated on nitrocellulose（NC）membrane,then sera were added to NC,and the color reaction was done with colloidal gold labeled to staphylococcal protein A（SPA）.The results showed that this method was simple,time effective,and the result was easy to judge.Furthermore,this method had a better specificity without cross-reaction with serum of the FMDV vaccinated pig and the positive sera of porcine parvovirus,hog cholera virus,porcine reproductive and respiratory syndrome,and pseudorabies virus.50 pig sera were examined by dot immunogold filtration assay（DIGFA）and ELISA,and the results showed that the positive rate of DIGFA was 6%（9/150）,and the ELISA＇s was 5.3%（8/150）,and the coincidence of them was 99.3%.In conclusion,the developed DIGFA could be applied for rapid detection of 3AB antibody against FMDV in animals.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2010年第7期717-721,共5页
Chinese Veterinary Science
基金
国家质检质量监督检验检疫总局科研项目(2007IK039)
