摘要
目的 探讨缺氧诱导因子及其相关蛋白在肝母细胞瘤（hepatoblastoma，HB）中表达情况及其作用。方法选择1998年至2009年人住我院并接受手术的肝母细胞瘤患儿及胆道闭锁患儿各30例，分为三组：肝母细胞瘤肿瘤组织、距肿瘤组织边缘5cm以外的正常肝脏组织以及胆道闭锁活检肝组织，肿瘤组中以PRETEXT分期系统，将肿瘤组分为低分组（Ⅰ组＋Ⅱ组，10例）和高分组（Ⅲ组＋Ⅳ组，20例）。采用免疫组织化学染色方法检测HIF-1Q、HIF-2α、VEGF、GLUT-1、TG-α在上述标本中的表达情况，并且比较各组的表达情况差异。结果5种蛋白的阳性表达均主要表现为细胞质内棕黄色颗粒。肿瘤组HIF-1α、HIF-2α、VEGF、GLUT-1、TGFα表达明显高于其他两组（P〈0．01）。肿瘤高分组中HIF1d及TGF-α的表达水平高于低分组（P〈0．05），而HIF-2α、VEGF、GLUT-1的表达两者无显著差别。结论缺氧诱导因子及其相关蛋白在肝母细胞瘤中的表达均有增加，提示HIF可能在肝母细胞瘤的发生发展中起着重要作用，其中HIF-1a还可能是潜在的预后判断指标之一。
Objective To investigate the expressions of hypoxia-inducible factors （HIF） and its related proteins in hepatoblastoma. Methods Between 1998 and 2009, 30 patients who were diagnosed with hepatoblastoma and 30 patients with biliary atresia were recruited in this study. The specimens of the tumor group were collected from the hepatoblastoma tissue. The specimens of the first control group were the liver tissues which located more than 5 cm away/tom the borderline of the tumor. The specimens of the second control group were the liver tissues from the 30 patients with biliary atresia. Immunohistochemical staining was performed to examine the expression level of HIF-1α, HIF-2a, vascular endothelial growth factor （VEGF）, glucose transporter-I （GT1）, and transforming growth factoralpha （TGF-α） in specimens. Results The positive stained proteins appeared as brown granules in cytoplasm. The expression levels of all the 5 proteins of the tumor group were significantly higher than those of the other two control groups （P〈0. 01）. The expression level of HIF-1α and TGF-α of the high-grade tumors were significantly higher than those of the low-grade tumors（P〈 0. 05）. Conclusions The expressions of HIF-1a, HIF 2a, VEGF, GT1 and TGF-α increase in hepatoblastoma tissues. It indicates HIF may play a role in the carcinogenesis of hepatoblastoma.
出处
《中华小儿外科杂志》
CSCD
北大核心
2010年第7期485-489,共5页
Chinese Journal of Pediatric Surgery
关键词
缺氧诱导因子
肝母细胞瘤
免疫组织化学
Hypoxia-inducible factor
Hepatoblastoma
Immunohistochemical staining
