摘要
利用PCR-DGGE技术研究刺参(Apostichopus japonicus)前肠、中肠和后肠内含物的细菌群落组成。通过软件Bio-rad Quantity one分析DGGE指纹图谱,发现刺参后肠内含物的条带数目显著高于前肠和中肠(P=0.003,P=0.016),表明刺参后肠内含物的细菌多样性最高,其次是中肠;前肠内含物的细菌多样性最低。UPGMA聚类分析发现,不同刺参个体其后肠的细菌群落组成差异最小,前肠的细菌群落差异最大。经DGGE分离、条带切割和序列测定,共获得了13条序列,系统发育分析表明,刺参消化道内含物的细菌群落可主要归属于5大类群,即α-变形菌纲(α-proteobacteria)、γ-变形菌纲(γ-proteobacteria)、δ-变形菌纲(δ-proteobacteria)、拟杆菌纲(Bacteroidetes)和柔膜菌纲(Mollicutes)。刺参前肠、中肠和后肠内含物的优势菌群均为γ-proteobacteria。Blast分析显示,其中12条与之亲缘关系最近的序列来自从海洋环境中获得的细菌克隆,表明刺参消化道的细菌群落可能直接或间接来源于刺参的栖息地环境。
The bacterial community composition in gut contents,including anterior intestine,middle intestine and posterior intestine of sea cucumber（ Apostichopus japonicus）,was studied by 16S rDNA PCR denaturing gradient gel electrophoresis（ PCR-DGGE）. The band numbers in posterior intestine were significantly higher than those in anterior intestine and middle intestine（ P = 0.003,P = 0.016,respectively）,which showed that the bacterial diversity in posterior intestine was higher than that in anterior intestine and middle intestine. UPMGA cluster analysis revealed that the bacterial community in posterior intestine had higher similarity than that in anterior intestine and middle intestine among different individuals. Phylogenetic analysis of 13 cloned bands showed that bacterial phylotypes were closely related to α-proteobacteria,γ-proteobacteria,δ-proteobacteria,bacteroidetes and mollicutes,and γ-proteobacteria were the dominant bacteria in the three parts of the digestive tract in A. japonicus. The closest relative bacteria of 12 cloned bands in the present study were from marine environment,which suggested that the bacteria in the gut contents of A. japonicus were from the surrounding habitat environment.
出处
《中国水产科学》
CAS
CSCD
北大核心
2010年第4期671-680,共10页
Journal of Fishery Sciences of China
基金
国家自然科学基金资助项目(40906071)
中国博士后科学基金资助项目(20090451355)
中国水产科学研究院黄海水产研究所基本科研业务经费资助项目(2009-ts-07)
