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嗅鞘细胞移植对大鼠脊髓损伤的修复作用及机制

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摘要 目的探讨嗅鞘细胞(OECs)移植对损伤脊髓的作用及机制。方法取48只成年SD大鼠制备脊髓损伤模型,造模成功后将大鼠随机分为两组各24只,OECs组行OECs移植:取10μl的OECs悬液于脊髓损伤处及损伤段远、近端蛛网膜下腔分别注入8、1、1μl,并各留针2 min,缓慢退针;对照组予生理盐水10μl注入,其他操作同观察组。应用BBB运动功能评分比较两组行为学指标,HE染色、胶原纤维酸性蛋白(GFAP)免疫组化染色分别观察脊髓损伤组织学变化及T9星形胶质细胞增生情况。结果干预后2～8周OECs组运动功能恢复情况、BBB评分均显著高于对照组及干预前,P均<0.01。干预后4周HE染色示对照组脊髓组织有较多空腔,神经纤维及细胞坏死,颜色较暗;而OECs组脊髓组织中无明显空腔,空泡小而少,色泽较亮。GFAP免疫组化染色示两组受损脊髓节段均出现星形胶质细胞反应性增生,但OECs组明显轻于对照组。结论 OECs对损伤脊髓具有修复和保护作用,其机制为促进损伤脊髓的轴突再生,减少星形胶质细胞增生。
作者 梁秋冬 董玉珍 施辛革 段永壮
机构地区 新乡医学院第一附属医院 郑州大学第一附属医院
出处 《山东医药》 CAS 北大核心 2010年第27期32-34,共3页 Shandong Medical Journal
基金 河南省科技厅资助项目(082102310083)
关键词 嗅鞘细胞 轴突再生 脊髓损伤
分类号 R651.2 [医药卫生—外科学]
  • 相关文献

参考文献11

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二级参考文献9

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山东医药
2010年 第27期

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