摘要
探讨了BTH(苯并噻重氮)对采后杧果(Mangifera indica Linn.cv.Hongmang No.6)果实抗病性诱导的效果及其对几种抗病相关酶活性的影响。用质量浓度为50mg·L-1的BTH水溶液对杧果果实作喷雾处理后,将杧果贮藏于(20±1)℃,相对湿度为80%的恒温箱内,观察贮藏过程中杧果发病情况和防御酶活性的变化。结果表明,BTH处理不仅能显著降低杧果果实自然发病的病情指数,而且明显提高了过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)和β-1,3-葡聚糖酶(GUN)等抗病相关酶的活性。此外,BTH处理也提高了过氧化氢(H2O2)和总酚的含量,降低了丙二醛(MDA)的含量。因此,BTH处理提高采后杧果果实的抗病性可能是通过激活杧果的防御系统而发挥作用。
The effects of benzothiadiazole (BTH) on induction of disease resistance in harvested mango (Mangifera indica Linn. cv. Hongmang No.6)fruits and the activities of several defense-related enzymes were investigated. Harvested mango fruits were sprayed with 50 mg·L-1 of BTH solution before being stored at (20±1) ℃ with the relative humidity of 80%. The disease incidence and five defense-related enzymes were monitored during the storage. The results showed that, compared with the control, BTH treatment significantly reduced the disease indices of mango fruits. Moreover, BTH treatment enhanced the activities of the defense-related enzymes, i.e., peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and β-1,3-glucanase (GUN). In addition, BTH treatment increased the contents of hydrogen peroxide (H2O2) and total phenolics, and decreased content of malondialdehyde (MDA). Therefore, the results suggested that the enhanced disease resistance in harvested mango by BTH treatment could result from the activation of the disease-related defense system.
出处
《果树学报》
CAS
CSCD
北大核心
2010年第4期585-590,共6页
Journal of Fruit Science
基金
农业科技成果转化资金项目(2006GB23260382)
中央级公益性科研院所基本科研业务费专项(中国热带农业科学院南亚热带作物研究所)
海南省热带园艺产品采后生理与保鲜重点实验室开放基金
十一五科技部支撑项目(2006BAD22B)
关键词
杧果
苯并噻重氮
防御酶
抗病性
Mango
Benzothiadiazole
Defense enzymes
Disease resistance