摘要
目的探讨急性冠状动脉综合征患者单核细胞中过氧化体增殖物激活型受体δ的表达水平与血清转化生长因子β1和白细胞介素1β水平的相关性。方法纳入55例急性冠状动脉综合征患者和47例健康对照者,分离其周围血单核细胞,应用逆转录聚合酶链反应法检测过氧化体增殖物激活型受体δmRNA的表达;应用酶联免疫吸附试验检测血清转化生长因子β1和白细胞介素1β的水平。结果急性冠状动脉综合征患者血单核细胞中过氧化体增殖物激活型受体δmRNA的表达水平显著低于健康对照组(0.13±0.09比0.34±0.08,P<0.01);血清白细胞介素1β水平高于健康对照组(142.7±39.3 ng/L比81.5±23.9 ng/L,P<0.01),而转化生长因子β1低于健康对照组(11.4±4.6μg/L比26.3±10.5μg/L,P<0.01);过氧化体增殖物激活型受体δmRNA表达水平与白细胞介素1β呈负相关(r=-0.437,P<0.05),与转化生长因子β1呈正相关(r=0.598,P<0.05)。结论过氧化体增殖物激活型受体δ可能在急性冠状动脉综合征的发病中具有重要作用。
Aim To evaluate the correlation among the level of peroxisome proliferator-activated receptors δ（PPARδ） expression in peripheral blood monocytes,serum levels of transforming growth factor-β1（TGF-β1） and interleukin-1β（IL-1β） in patients with acute coronary syndrome（ACS）. Methods Peripheral blood monocytes were collected from 55 patients with ACS and 47 healthy controls.The PPARδ mRNA expression was detected by reverse transcription polymerase chain reaction（RT-PCR）,while the serum level of TGF-β1 and IL-1β were measured by enzyme linked immunosorbent assay（ELISA）. Results The PPARδ mRNA expression level（0.13±0.09）was markedly reduced in peripheral blood monocytes from patients with ACS compared with healthy controls（0.34±0.08,P0.01）.The serum IL-1β level（142.7±39.3 ng/L）was significant higher while the TGF-β1 level（11.4±4.6 μg/L） was obvious lower in patients with ACS than controls（81.5±23.9 ng/L,26.3±10.5 μg/L）（both P0.01）.Furthermore,the PPARδ mRNA expression level was negatively correlated to IL-1β level（r=-0.437,P0.05） and positively related to TGF-β1 level（r=0.598,P0.05）. Conclusion The PPARδ might play a critical role in the pathogenesis of ACS.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2010年第5期401-404,共4页
Chinese Journal of Arteriosclerosis
关键词
冠状动脉疾病
单核细胞
基因表达
黏附分子
Coronary Artery Disease
Monocytes
Gene Expression
Adhesion Molecule
