摘要
背景:肌卫星细胞在骨骼肌的损伤、修复和维持中起着重要作用,骨骼肌的肌湿质量、肌横切面积及肌动蛋白的含量可为损伤肌肉的恢复提供指标。目的:观察骨骼肌慢肌卫星细胞移植对肌肉损伤的修复作用。方法:体外培养分离Sprague-Dawley大鼠慢肌卫星细胞,分别取慢肌卫星细胞悬液和生理盐水(对照组)注入钝挫伤动物模型的损伤部位,观察骨骼肌损伤的修复情况。结果与结论:移植后实验组比对照组恢复效果好。①卫星细胞移植后5,10,15d,实验组肌肉组织内荧光标记的肌卫星细胞逐渐减少,形成肌管并最终融合成肌纤维参与损伤修复。②移植后5,10,15d大鼠肌电图的纤颤电位和正尖波均逐渐减少,且损伤侧的肌电强度和波宽依次增大,逐渐接近正常侧,而对照组肌电强度与波宽则增加缓慢。③随着损伤修复的进程,肌湿质量恢复率均逐渐降低。④随着恢复的进程,实验组灰度值大于对照组数值。提示骨骼肌慢肌卫星细胞移植到钝挫损伤的肌肉内可明显加快损伤肌肉的恢复。
BACKGROUND：Muscle satellite cells play an important role in skeletal muscle injury,repair and maintenance.The muscle wet weight,muscle fiber cross-sectional area and the content of actin could provide indicators of the recovery of muscle injury.OBJECTIVE：To investigate the effect of transplantation of slow skeletal muscle satellite cells on repairing muscle contusion damage at 5,10,15 days post surgery.METHODS：Slow skeletal muscle satellite cells of Sprague-Dawley rat were cultured in vitro.Slow muscle satellite cells and physiological saline （control group） were respectively implanted into injured position of contusion animal model.The repairable conditions of skeletal muscle injury were observed.RESULTS AND CONCLUSION：The experimental group post-transplantation obtained good results compared with the control group.① At 5,10 and 15 days after the transplantation of satellite cells,satellite cells labeled with fluorescence in muscle tissue gradually reduced in the experimental group.Muscle satellite cells formed myotubes and eventually fused into muscle fibers so as to repair the damage.②At 5,10 and 15 days after transplantation,the fibrillation potentials and positive sharp waves were progressively reduced.Myoelectric intensity and pulse width in turn at the injury side were increased by degrees to near normal side,while EMG intensity and pulse width were increased slowly in the control group.③With the processing of the injured repair,the recovery rates of muscle wet weight were progressively reduced.④With the processing of the recovery,the gray values of the experimental group were higher than the control group.Results have indicated that the transplantation of slow skeletal muscle satellite cells into the contusion muscles can significantly improve the recovery of muscle contusion damage.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第23期4271-4274,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
南通大学大学生实践创新训练资助项目(2009042)
南通市科技局社会发展科技计划资助项目(S2008022)~~
