摘要
建立ＥＡＥ动物模型，检测脾细胞培养上清中γ干扰素（ＩＦＮγ）及白细胞介素４（ＩＬ４）水平。结果显示，实验性变态反应性脑脊髓炎（ＥＡＥ）组ＩＦＮγ浓度高于对照组（Ｐ＜０．０５），而ＩＬ４浓度低于对照组（Ｐ＜０．０１）。
To investigate the role of cytokines in pathogenesis of EAE, a model was established by injecting MBP into guinea pigs, a genetical susceptible animal. The animals were killed 8, 15 and 21 days after immunization respectively. Spleen single cell suspention was prepared. After cell culture, supernatants were assayed for the concentration of IFN γ and IL 4. The results showed that the levels of IFN γ in EAE model group were higher than those in control group ( P<0.05 ). While the levels of IL 4 in EAE model group were lower than those in control group ( P <0.01). The studies indicate that IFN γ and IL 4 contribute to the pathogenesis of EAE.
出处
《哈尔滨医科大学学报》
CAS
1999年第1期12-13,共2页
Journal of Harbin Medical University
关键词
变态反应性
脑脊髓炎
Γ-干扰素
IL-4
Experimental allergic encephalomyelitis
Interferon γ
Interleukin 4
Guinea pig
