摘要
用酶解法分离单个豚鼠心室肌细胞，以荧光探针Ｆｌｕｏ－３／ＡＭ标记胞浆内游离钙离子，用激光扫描共聚焦显微镜实时测定胞浆内［Ｃａ２＋］ｉ变化。分别观察了在正常台氏液和无钙台氏液中３０ｍｍｏｌ／ＬＫＣｌ除极对胞浆内［Ｃａ２＋］ｉ的影响。结果表明，在正常台氏液中，３０ｍｍｏｌ／ＬＫＣｌ除极可引起胞浆内［Ｃａ２＋］ｉ持续升高，持续时间超过２５０ｓ；在无钙台氏液中，３０ｍｍｏｌ／ＬＫＣｌ除极可引起胞浆内［Ｃａ２＋］ｉ短暂升高，持续时间约１００ｓ。由此可见，在豚鼠心室肌细胞上，ＫＣｌ除极可引起胞内钙贮库的钙释放，而且这种钙释放不需要钙内流的介导。胞浆内［Ｃａ２＋］ｉ的持续性升高需要外钙内流来维持。
To investigate the relationship between the membrane depolarization and the release of intracellular Ca 2+ stores, single cardiac ventricular myocytes of guinea pig were isolated by an enzymatic technique and loaded with the Ca 2+ indicator Fluo 3/AM. The changes of cytosolic [Ca 2+ ] i of ventricular myocytes depolarized by 30mmol/L KCl in normal Tyrode solution and Ca 2+ free Tyrode solution were observed by using laser scanning confocal microscope. Results showed that depolarization by 30mmol/L KCl caused constant increase of cytosolic [Ca 2+ ] i during 250s in normal Tyrode solution and transient increase in Ca 2+ free Tyrode solution. We concluded that depolarization caused the Ca 2+ release from intracellular Ca 2+ stores and Ca 2+ influx was not required to mediate, the constant increase of cytosolic [Ca 2+ ] i was sustained by Ca 2+ influx.
出处
《哈尔滨医科大学学报》
CAS
1999年第1期9-11,共3页
Journal of Harbin Medical University
基金
卫生部优秀青年科学基金
