摘要
目的：探讨自制的含组氨酸、Ｂｉｍａｋａｌｉｍ的细胞内液型胶体液（ＨＢＳ）对离体心脏的保存作用。方法：健康雄性ＳＤ大鼠１６只，体重２４０～２６０ｇ。随机均分为两组，采用微量灌注技术，分别用ＨＢＳ和托马斯停搏液（ＳＴＳ）４℃保存离体鼠心２４小时，再灌注６０分钟进行比较研究。结果：ＨＢＳ组心功能恢复程度明显优于ＳＴＳ组，再灌注期ＬＤＨ、ＥＴ释放量ＨＢＳ组低于ＳＴＳ组，ＨＢＳ组心肌ＡＴＰ含量高于ＳＴＳ组两倍以上，心肌超微结构保持完好。结论：ＨＢＳ低温保存鼠心２４小时效果明显优于临床上常用的ＳＴＳ。
Aim:How to prolong the safe preservation time of the donor heart for heart transplantation remains an important problem .For this reason,HBS was prepared to according to our own recipe.This study was to compared the protective and preservation effects on HBS with that of St.Thomas solution II(STS) on rat heart using Langendorff apparatus.Material and Method:16 SD rats were divided into two groups randomly.After arrestment,the isolated isovolumetric buffer perfused rat hearts were microperfused with the STS(n=8) and HBS(n=8) at 4℃ for 24 hours,then reperfused with K H solution for 1 hours.Results:Significant differences were observed between two groups:(1)Better recovery of left ventricular function in HBS group(P<0.05).(2)After reperfusion,the release of LDH and ET increased,but the LDH and ET concentration in group HBS were significantly lower than group STS(P<0.05).(3)The ATP content of myocardium was markedly higher in group HBS than group STS(P<0.05).(4)The ultra structural changes of myocardium in group HBS were milder than that in group STS.Conclusion:HBS is better than STS cardioplegic solution in myocardial protection and heart preservation in rats.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
1999年第1期51-54,共4页
Chinese Journal of Thoracic and Cardiovascular Surgery
关键词
微流量灌注
心脏保存液
托马斯停搏液
细胞内液
Heart preservation Myocardial protection Histidine Bimakalim solution St.Thomas solution
