摘要
[Objective] The aim was to study response of chlorophyll fluorescence parameters in Trifolium repens L.leaves to water stress from perspective of physiology.[Method] With T.repens cultivar ＂Haifa＂ as tested material,three soil water content levels were set to culture plants,including 75% （no stress,CK）,50% （moderate stress,LD） and 25% （severe stress,HD）,the effects of water stress on chlorophyll fluorescence parameters were determined.[Result] The results showed that the chlorophyll fluorescence parameters changed a little when the relative soil moisture content was 75% and 50%,while the chlorophyll fluorescence parameters such as conversion efficiency of primary light energy （Fv/Fm） of PS Ⅱ,the photochemical quenching coefficient （qP） and the quantum yield of PS Ⅱ electron transport （φPS Ⅱ） decreased respectively when the relative soil moisture content was 25%.[Conclusion] When T.repens in severe drought conditions,the physiological functions were damaged,showing symptoms of drought injury.
[目的]从生理角度研究白三叶(Trifolium repens)叶片叶绿素荧光参数对水分胁迫的响应。[方法]以白三叶品种"海发"为供试材料,设置75%(无胁迫,CK)、50%(轻度胁迫,LD)和25%(重度胁迫,HD)3个土壤含水量水平对植株进行培养,测定水分胁迫对一系列叶绿素荧光参数的影响,并进行统计学分析。[结果]土壤相对含水量为75%和50%时,白三叶叶片叶绿素荧光参数受到的影响不大,说明白三叶在这2种水分条件下能够正常生长;土壤相对含水量为25%时,初始荧光后极显著高于CK和LD处理,原初光能转化效率(Fv/Fm)、PSⅡ电子传递量子产量(φPSⅡ)和光化学猝灭系数显著降低。[结论]白三叶在重度干旱(HD)条件下,生理机能遭到破坏,不能正常生长,表现出旱害症状。
基金
Supported by Doctor Foundation of Shanghai Institute of Technology~~
