摘要
目的检测帕金森病（ＰＤ）模型猴双侧尾核细胞外液兴奋性氨基酸（ＥＡＡ）含量的变化，探讨ＥＡＡ在ＰＤ发病机制中的作用。方法应用１－甲基－４－苯基－１，２，３，６－四氢吡啶（ＭＰＴＰ）经一侧颈总动脉注射制备偏侧ＰＤ猴模型（共４只），模型制备后３．５年，根据模型猴运动症状及对阿朴吗啡（ＡＰＯ）诱发旋转行为的反应，将模型猴分为模型组（２只）及模型恢复组（２只），采用颅内微透析技术结合反相高效液相色谱－荧光法在体检测猴双侧尾核细胞外液ＥＡＡ及抑制性氨基酸（ＩＡＡ）含量。结果模型组中ＭＰＴＰ损毁侧尾核细胞外液谷氨酸（Ｇｌｕ）、天门冬氨酸（Ａｓｐ）、甘氨酸（Ｇｌｙ）、γ－氨基丁酸（γ－ＧＡＢＡ）较未损毁侧分别增加了２．４、７．２、１．３及５．０倍；而在模型恢复组中ＭＰＴＰ损毁侧Ｇｌｕ、Ａｓｐ、Ｇｌｙ亦较未损毁侧增高，但增加不明显，γ－ＧＡＢＡ含量相差不大。结论尾核部位Ｇｌｕ、Ａｓｐ增高可能与ＰＤ猴的运动症状的产生相关，Ｇｌｕ。
Objective To investigate the role of excitatory amino acid (EAA) in pathogenesis of Parkinson's disease by measuring the level of EAA in parkinsonian monkey models. Methods Four monkey models of hemiparkinsonism were established by unilateral intracarotid infusion of 1 methyl 4 phenyl 1,2,3,6 tetrahydropyridine (MPTP). Three and half years later, according to their response to apomorphine, the monkeys were divided into 2 groups, the model group (2 monkeys) and the model reversible group (2 monkeys). The concentrations of EAA and inhibitory amino acid (IAA) in the bilateral caudate nuclei of the monkeys were measured by brain microdialysis combined with reverse phase high performance liquid chromatography (RP HPLC) and fluorometric detection. Results In the model group, the contents of Glu, Asp, Gly and γ GABA in the MPTP treated side were increased by 2 4, 7 2, 1 3 and 5 0 times, respectively, as compared with those in the untreated side. In the model reversible group, the levels of Glu, Asp and Gly in the MPTP treated side were slightly elevated as compared with those in the untreated side, and the content of γ GABA was not enhanced. Conclusions The levels of EAA, Glu and Asp may be related to the symptoms of parkinsonian monkey models. Glu and Asp may participate in the damage of striatal by MPTP.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
1999年第1期45-47,共3页
Chinese Journal of Geriatrics
基金
上海市卫生系统百名跨世纪优秀学科接班人计划
上海市高教局基金
