摘要
为了探讨影响微生物菌丝球生长的物理化学因素和控制菌丝球大小的规律以及微生物吸附重金属的效果，对黄孢展齿革菌（Ｐｈａｎｅｒｏｃｈａｅｔｅｃｈｒｙｓｏｓｐｏｒｉｕｍ）呈球状体生长和用此菌丝球吸附水溶液中的Ｐｂ２＋进行了研究．实验结果表明，在培养液ｐＨ值为４．５，孢子悬液浓度为１０６个／ｍｌ，表面活性剂吐温８０的浓度为０．１％，高碳氮和摇床转速为１５０ｒ／ｍｉｎ的条件下，于３９℃下培养３ｄ，形成直径在１．５—１．７ｍｍ范围内的菌丝球，光滑均匀，具有一定机械强度，对Ｐｂ２＋的吸附能力最强．用０．２ｍｏｌ／Ｌ的ＮａＯＨ溶液处理该菌丝球，对２５ｍｇ／Ｌ的铅溶液的吸附率达到了９５％以上，表明用该菌丝球吸附水溶液中的Ｐｂ２＋是可行的．
The research on the growth of Phanerochaete chrysosporium in the form of pellet was made in this paper to examine the effects of physical and chemical conditions on the formation of mycelial pellets.The experimental results showed that the microorganism pellets of smooth surface,even size(diameter)and good mechanical properties could be obtained at the follwoing culture conditions:pH4 5,10 6 spores/ml in inoculum,0 1% Tween 80 in medium,high C/N and on the shaker of 150r/min for 3d.Using P.chrysosporium mycelial pellets in the biosorption of Pb 2+ from aqueous solution,the uptake reached the maximum when the diameter of pellet was in the range of 1 5—1 7mm.When the pellets were pretreated by boiling in 0 2mol/L NaOH for 40min,the Pb 2+ uptake was far higher than the pellets without pretreatment,and the removal efficiency could reach above 95%.The research results showed that the removal of Pb 2+ from aqueous solution by P.chrysosporium mycelial pellets was practical.
出处
《环境科学》
EI
CAS
CSCD
北大核心
1999年第1期33-38,共6页
Environmental Science
基金
国家教委留学回国人员科研启动基金
关键词
白腐真菌
菌丝球
生物吸附
吸附
铅
废水处理
Phanerochaete chrysosporium ,mycelial pellet,biosorption,Pb 2+ ,C/N,biomass,spore suspension,surface active agent.
