摘要
目的研究从黄连须根中分离纯化黄连生物碱单体和总碱的联产工艺。方法用0.3%硫酸水加热回流提取黄连须中总生物碱,用石灰乳调节提取液pH为5～6,过滤;滤液用JG-101树脂吸附,用不同洗脱剂分步洗脱;重结晶获得药根碱和小檗碱单体,盐析获得黄连总生物碱;用HPLC分析黄连生物碱及总碱含量。结果用2%氢氧化钠洗脱,酸中和后重结晶得药根碱单体;再用70%酸性甲醇(1%H2SO4)溶液洗脱总生物碱;洗脱液中加入2%NaCl沉淀并重结晶得小糪碱单体;上清液加20%NaCl沉淀获得余下总生物碱。结论用JG-101树脂能够很好吸附黄连总生物碱,药根碱和小檗碱单体的纯度均达到95%以上,总生物碱(小檗碱、黄连碱、巴马汀和药根碱之和)的含量为90%左右。
Objective To investigate the joint producing technology by isolating and purifying the total alkaloids and monomeric alkaloids from the fibrous root of Coptis chinensis Franch.Methods The extract of total alkaloids was obtained from the fibrous root of Coptis chinensis Franch with 0.3% H2SO4 by heating reflux and precipitated by adjusting pH to 56 with lime.After filtration,the filtrate was absorbed with JG-101 resin and eluted with different eluant.Subsequently,jatrorrhizine and berberine were obtained by recrystallized,and total alkaloids by salting out.The content of alkaloids was analyzed by HPLC.Results Jatrorrhizine faction was eluted with 2% NaOH,neutralized with HCl,fruther recrystallized in distilled water to obtain jatrorrhizine monomer.Total alkaloids were eluted with 70% acidic methanol（containing 10% H2SO4）,from which berberine was obtained by 2% NaCl precipitation and recrystallization.Supernatant was treated with the mixture of 20% NaCl to collect the residual total alk...更多aloids.Conclusion JG-101 resin has a strong absorption capability to total alkaloids.The purity of jatrorrhizine and berberine can be up to 95%,and that of total alkaloids（berberine,coptisine,palmatine and jateorrhizine）is about 90%. 还原
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第7期1644-1646,共3页
Lishizhen Medicine and Materia Medica Research
基金
重庆市科委中药创制重大专项(No.CSTC
2008AA5021)
重庆市自然科学基金(No.CSTC
2008BB5257)
关键词
黄连
须根
总生物碱
生物碱单体
联产工艺
树脂
Coptis chinensis Franch,Fibrous root,Total alkaloids,Alkaloid monomer,Joint producing technology,Resin
