摘要
目的 研究家族成员中反复腹痛和无腹痛者的幽门螺杆菌（Helicobacter priori,Hp）感染和人类白细胞抗原（HLA）-DQA1等位基因频率分布.方法 将反复腹痛患儿为核心的20个家庭118名同代、一级和二级亲属分为反复腹痛组和无腹痛组,应用胶体金标免疫渗滤法和免疫印迹法检测118名家族成员血Hp-IgG抗体和亚型.用聚合酶链反应-序列特异性引物（PCR-SSP）方法对2组进行HLA-DQA1等位基因分型.样本的分布经过Hardy-Weinberg平衡检验,P〉0.05,组间HLA-DQA1等位基因比较采用四格表X2检验.结果 20个家族中Hp抗体和Hp亚型阳性率分别为100%和96.6%,Ⅰ型Hp感染占55.1%（65/118）,Ⅱ型Hp感染为41.5%（49/118）.反复腹痛组HLADQA1＊0302的等位基因频率明显高于无腹痛组,2组比较差异有统计学意义（23%vs.2%,X2=13.277,P=0.000）.结论 在Hp感染的家族成员中,反复腹痛者与无腹痛者之间存在免疫遗传学差异,HLA-DQA1＊0302可能是Hp感染后反复腹痛的相关基因.
Objective To investigate Helicobacter pylori（Hp）infection and HLA-DQA1 allelic frequency in family members of children with recurrent abdominal pain.Methods One hundred and eighteen family members of 20 children with recurrent abdominal pain were divided into two groups：with and without recurrent abdominal pain.Serum Hp antibody was tested by dot immunogold filtration assay and immunophenotyping was determined by Western blot（immunobiot）technique.Polymerase chain reactionsequence specific primers（PCR-SSP）technique Was applied to identify HLA-DQAi allelic frequencies.Hardy-Weinberg equilibrium test was performed（P〉0.05）,and Chi-square test was used to compare the frequency of HLA-DQA1 alleles between the groups.Results The Hp seropositive rate in 118 members Was 100%and the Hp immunophenotyping was 96.6%.The prevalence of Hp Ⅰ and Ⅱ type was 55.1%（65/118）and41.5%（49/118）.HLA-DQA1＊0302 allelic frequency Was significantly higher in subjects with recurrent abdominal pain than that in subjects without one（23%vs.2%,X2=13.277,P=0.000）.Conclusion There is immunogenetic difference between familial members with and without recurrent abdominal pain infected by Hp,and HLA-DQA1＊0302 may be the associated gene contributing to different clinical outcomes after Hp infections.
出处
《中华临床感染病杂志》
CAS
2010年第3期166-169,共4页
Chinese Journal of Clinical Infectious Diseases
基金
云南省自然科学基金（2009CD163）
云南省中青年学术技术带头人后备人才培养计划资助项目（2006PY1-18）
