摘要
目的：重组人胰岛素样生长因子－Ｉ（ｒｈＩＧＦ－Ｉ）、重组人骨形态发生蛋白－２（ｒｈＢＭＰ－２）分别或联合应用对人牙周膜（ＰＤＬ）细胞增殖的影响。方法：采用组织块法体外培养人ＰＤＬ细胞，ＭＴＴ法测定ＰＤＬ细胞在不同生长因子刺激下的增殖情况。结果：ｒｈＩＧＦ－Ｉ、ｒｈＢＭＰ－２都可促进人ＰＤＬ细胞的增殖，这种促增殖作用呈一定的浓度依赖性，ｒｈＩＧＦ－Ｉ与ｒｈＢＭＰ－２联合应用对人ＰＤＬ细胞的增殖有协同作用，且与单独应用相比相差显著。结论：ｒｈＩＧＦ－Ｉ、ｒｈＢＭＰ－２可望作为牙周再生的生物活性介质，ｒｈＩＧＦ－Ｉ与ｒｈＢＭＰ－２联合应用对ＰＤＬ细胞的促增殖作用更强。
Aim: To study the effect of recombinant human Insulin-like growth factor-I and recombinant human bone morphogenetic protein-2 on human periodontal ligament cells proliferation. Methods: Human periodontal ligament cells were cultured by tissue explant and its proliferation were mesured by MTT colorimetric assay. Results: rhIGF-I or rhBMP-2 can stimulate human PDL cells proliferation in dose-dependent manner. The co-ordinate use of rhIGF-I and rhBMP-2 has synergetic effect on PDL cells proliferation. Conclusions: rhIGF-I and rhBMP-2 can be used as bioactive mediator on periodontal regeneration.
出处
《牙体牙髓牙周病学杂志》
CAS
1999年第1期31-33,共3页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金
关键词
牙周膜细胞
细胞培养
胰岛素样
生长因子
RHBMP2
periodontal ligament cells
cell culture
recombinant human insulin-like growth factor-I
recombinant human bone morphogenetic protein-2
