摘要
目的：优化体外人牙髓细胞（ＨＤＰＣ）培养条件，以提高ＨＤＰＣ原代培养成功率。方法：采用玻璃及塑料培养瓶、高糖ＤＭＥＭ以及标准型、合格型胎牛血清，分别比较原代牙髓组织细胞游出率。结果：塑料培养瓶细胞游出率以及细胞游出最短时间均明显优于常用玻璃培养瓶；两种不同型号胎牛血清比较为标准型胎牛血清的组织块贴壁以及细胞游出率均优于合格型胎牛血清；高糖ＤＭＥＭ适合于ＨＰＤＣ培养。结论：商品塑料培养瓶、标准胎牛血清、高糖ＤＭＥＭ是提高体外ＨＤＰＣ培养成功率的关键。
Aim:To enhance the success rate of primary pulp tissues, we established the excellent condition suitable for culture human dental pulp cells in vitro . Methods: The cell growth rate of pulp tissues was compared with plastic culture bottle against glass one; qualified fetal bovine serium (QFBS) contained in DMEM( high glucose) against certified fetal bovine serium(CFBS), respectively. Results: The cell growth rate of pulp tissues in plastic bottle was higher than that of the glass bottle as well as in CFBS higher than that in QFBS; DMEM in high glucose was suitable for HDPC. Conclusions: It was a key for HDPC primary culture using plastic bottle, DMEM in high glucose and CFBS.
出处
《牙体牙髓牙周病学杂志》
CAS
1999年第1期18-20,共3页
Chinese Journal of Conservative Dentistry
关键词
体外培养
人牙髓细胞
培养瓶
胎牛血清
高糖DMEM
culture in vitro
human dental pulp cells
culture bottle
fatal bovine serium
DMEM in high glucose
