摘要
为制备Ｍｒ小的葡激酶以便作口服制剂，用ＰＣＲ技术克隆Ｎ末端缺失１８个氨基酸的葡激酶突变体基因。经大肠杆菌表达，目的蛋白占总菌体蛋白的３２％。表达产物Ｍｒ约为１４０００，在菌体内形成包涵体。经复性后，该突变体对纤溶酶原具有激活作用。
Staphylokinase mutant gene,deleted 18 amino acids in N end was amplified from staphylokinase gene,and ligated into pUC19 vector,then recombinated with PLY 4, an expression vector,and transformed E.coli. The expression product of mutant gene was 32% of total E.coli protein,and formed inclusion body.The M r of staphylokinase mutant is 14 000. The mutant has the same immunoreactivity as staphylokinase.
出处
《药物生物技术》
CAS
CSCD
1999年第1期9-13,共5页
Pharmaceutical Biotechnology
