摘要
Objective： To investigate the improving effects of different moxibustion therapies on colonic injury in rats with ulcerative colitis （UC）, and on the expression of Bcl-2 and Bax mRNA.Methods： Rats model of UC was made with immune methods and local stimulation. The models were treated with different moxibustion therapies 14 times, and then the expression of Bcl-2 and BaxmRNA were tested in the colonic tissue with fluorescent quantitative PCR. Results： （1）General scores of colon and histologic scores of injury were improved in all treatment groups except the model group（P〈0.05, P〈0.01）; （2） The expression of Bcl-2 mRNA in the model rats of UC was higher than that in the normal rats （P〈0.01）, whereas Bax lower （P〈0.01）; （3） After treatments, the expression of Bcl-2mRNA was lower in the herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion groups than that in the model group （P〈0.05, P〈0.01）, and Bax mRNAin the herb cake-partitioned and ginger-partitioned groups was higher （P〈0.05）. Conclusion： Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, garlic-partitioned moxibustion, andmild moxibustion could improve the pathological injury in UC rats. Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion could inhibit theexpression of Bcl-2 mRNA and Bax mRNA in the colonic tissue of UC rats to regulate the ratio of Bcl-2/Bax mRNA.
目的：探讨不同灸法对溃疡性结肠炎（ulcerativecolitis，UC）大鼠结肠损伤的改善作用，以及对Bcl-2mRNA、BaxmRNA表达的影响。方法：采用免疫学方法加局部刺激制备实验性UC大鼠模型。造模后采用不同灸法治疗14次，实时荧光定量PCR技术检测结肠组织Bcl-2mRNA、BaxmRNA表达。结果：（1）各治疗组结肠大体形态损伤积分和光镜下组织学损伤积分较模型组比较有明显改善（Jp〈0．05，P〈0．01）：（2）uc模型大鼠结肠组织Bcl-2mRNA的表达显著高于正常大鼠沪〈0．01），而BaxmRNA的表达显著低于正常大鼠（P〈0．01）；（3）治疗后，隔药灸组、隔姜灸组、隔蒜灸组大鼠结肠组织Bcl．2mRNA的表达明显下调，与模型组比较有显著差异（P〈0．05或P〈0．01）；隔药灸组、隔姜灸组BaxmRNA的表达明显增高，与模型组比较有显著差异（P〈0．05）。结论：初步研究结果提示隔药灸、隔姜灸、隔蒜灸、温和灸治疗均能有效改善UC大鼠结肠病理损伤，隔药灸、隔姜灸、隔蒜灸可不同程度抑制UC大鼠结肠组织Bcl-2mRNA和BaxmRNA的表达，调节Bcl-2／BaxmRNA的比例关系。
