摘要
目的:对中药泽兰进行抗氧化活性的研究,并筛选其主要的活性部位。方法:采用高通量活性筛选技术,以清除DPPH和超氧阴离子自由基能力、还原Fe3+能力及抑制脂质过氧化能力为指标,评价泽兰乙醇总提取物及不同极性部位的抗氧化能力。结果:泽兰及其乙酸乙酯部位(LLE-A.E,MCCM2921)和正丁醇部位(LLE-A.B,MCCM2922)具有较强的清除DPPH(二苯代苦味肼)、超氧阴离子自由基能力、还原Fe3+能力和抑制脂质过氧化能力。结论:泽兰及其不同极性部位具有清除自由基作用,这可以用来解释其在传统应用中的活血化瘀、抗纤维化作用。
Objective： To study the antioxidant activity of Herba Lycopi,and screen the main active fractions.Method： The different polar fractions were isolated from the ethanol extract of Herba Lycopi.The scavenging activity to DPPH and superoxide anions,reducing effect on Fe3＋,and inhibiting activity of lipid peroxidation of the fractions were studied in vitro by high-throughput screening technique.Result： The ethanol extract,ethyl acetate fraction（LLE-A.E,MCCM2921） and n-buthanol fraction（LLE-A.B,MCCM2922） of Herba Lycopi showed the scavenging activity to DPPH and superoxide anions,reducing effect on Fe3＋,and inhibiting activity of lipid peroxidation.Conclusion： The ethanol extract and different polar fractions showed antioxidant capacity in vitro.That may be explain that the effect of activating blood and dissolving stasis and anti-fibrosis of Herba Lycopi in traditional application.The results may be helpful for further development of Herba Lycopi as a low-toxic antioxidant drug.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第13期1754-1757,共4页
China Journal of Chinese Materia Medica
关键词
泽兰
抗氧化活性
自由基
活性部位
Herba Lycopi
antioxidant
antioxidant
active fractions
